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Literature summary for 3.7.1.3 extracted from
- Properties of crystalline Kynureninase from Pseudomonas marginalis (1973), Biochemistry, 12, 2969-2974.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas marginalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-cycloserine | 1 mM | Pseudomonas marginalis |  |
| D-penicillamine | 1 mM | Pseudomonas marginalis | |
| HgCl2 | 1 mM | Pseudomonas marginalis | |
| KCN | 1 mM | Pseudomonas marginalis | |
| NH2OH | - |
Pseudomonas marginalis | |
| p-chloromercuribenzoate | 0.5 mM | Pseudomonas marginalis | |
| phenylhydrazine-HCl | 1 mM | Pseudomonas marginalis | |
| Semicarbazide | 1 mM | Pseudomonas marginalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
L-kynurenine | - |
Pseudomonas marginalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 91000 | - |
gel filtration | Pseudomonas marginalis |
| 100000 | - |
sedimentation equilibrium method | Pseudomonas marginalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-kynurenine + H2O | Pseudomonas marginalis | - |
anthranilate + L-alanine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas marginalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas marginalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, phosphate buffer, pH 7.2, 0.02 mM pyridoxal-5'-phosphate, 1 week | Pseudomonas marginalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-kynurenine + H2O | - |
Pseudomonas marginalis | anthranilate + L-alanine | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas marginalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8 | - |
Pseudomonas marginalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Pseudomonas marginalis | |
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