Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate | at pH 7.0 and 60°C | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. LD2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity column chromatography | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate + H2O | - |
Pseudomonas sp. | 2-hydroxypenta-2,4-dienoate + benzoate | - |
? | |
2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate + H2O | - |
Pseudomonas sp. LD2 | 2-hydroxypenta-2,4-dienoate + benzoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-oxo-6(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase | - |
Pseudomonas sp. |
HOPDA hydrolase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | the enzyme exhibits activity between pH 6.5 and 10.5 | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme has no cofactor requirement | Pseudomonas sp. |