Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-diacetylphloroglucinol + H2O | Pseudomonas fluorescens | - |
2-acetylphloroglucinol + acetate | - |
? | |
2,4-diacetylphloroglucinol + H2O | Pseudomonas fluorescens | degradation of 2,4-diacetylphloroglucinol catalyzed by PhlG plays an insignificant role in 2,4-diacetylphloroglucinol tolerance but contributes to bacterial growth advantages under carbon/nitrogen starvation conditions | 2-acetylphloroglucinol + acetate | - |
? | |
2,4-diacetylphloroglucinol + H2O | Pseudomonas fluorescens 2P24 | - |
2-acetylphloroglucinol + acetate | - |
? | |
2,4-diacetylphloroglucinol + H2O | Pseudomonas fluorescens 2P24 | degradation of 2,4-diacetylphloroglucinol catalyzed by PhlG plays an insignificant role in 2,4-diacetylphloroglucinol tolerance but contributes to bacterial growth advantages under carbon/nitrogen starvation conditions | 2-acetylphloroglucinol + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens | Q4JIX4 | - |
- |
Pseudomonas fluorescens 2P24 | - |
- |
- |
Pseudomonas fluorescens 2P24 | Q4JIX4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-diacetylphloroglucinol + H2O | - |
Pseudomonas fluorescens | 2-acetylphloroglucinol + acetate | - |
? | |
2,4-diacetylphloroglucinol + H2O | degradation of 2,4-diacetylphloroglucinol catalyzed by PhlG plays an insignificant role in 2,4-diacetylphloroglucinol tolerance but contributes to bacterial growth advantages under carbon/nitrogen starvation conditions | Pseudomonas fluorescens | 2-acetylphloroglucinol + acetate | - |
? | |
2,4-diacetylphloroglucinol + H2O | - |
Pseudomonas fluorescens 2P24 | 2-acetylphloroglucinol + acetate | - |
? | |
2,4-diacetylphloroglucinol + H2O | degradation of 2,4-diacetylphloroglucinol catalyzed by PhlG plays an insignificant role in 2,4-diacetylphloroglucinol tolerance but contributes to bacterial growth advantages under carbon/nitrogen starvation conditions | Pseudomonas fluorescens 2P24 | 2-acetylphloroglucinol + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,4-DAPG hydrolase | - |
Pseudomonas fluorescens |
Phlg | - |
Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Pseudomonas fluorescens |
Organism | Comment | Expression |
---|---|---|
Pseudomonas fluorescens | the transcriptional regulator PhlH represses the expression of the enzyme | down |
Pseudomonas fluorescens | the pathway-specific regulator PhlH represses the phlG gene expression in vivo.Repressed expression of phlG is dependent on the interaction between PhlH and the palindromic sequence within the phlG promoter region | down |
Pseudomonas fluorescens | at 8 h after inoculation, 0.1 mM 2,4-diacetylphloroglucinol has an about 8fold induction of enzyme expression, whereas 0.1 mM monoacetylphloroglucinol has about 2fold induction of enzyme expression | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme contributes to bacterial growth advantages under carbon/nitrogen starvation conditions | Pseudomonas fluorescens |
physiological function | the pathway-specific regulator PhlH modulates 2,4-diacetylphloroglucinol levels by controlling the expression of 2,4-diacetylphloroglucinol hydrolase PhlG in response to 2,4-diacetylphloroglucinol and monoacetylphloroglucinol. Since 2,4-diacetylphloroglucinol biosynthesis imposes a metabolic burden on biocontrol pseudomonads, it is expected that the fine regulation of phlG by PhlH offers a way to dynamically modulate the metabolic loads attributed to 2,4-diacetylphloroglucinol production | Pseudomonas fluorescens |