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Literature summary for 3.7.1.24 extracted from

  • Saitou, H.; Watanabe, M.; Maruyama, K.
    Molecular and catalytic properties of 2,4-diacetylphloroglucinol hydrolase (PhlG) from Pseudomonas sp. YGJ3 (2012), Biosci. Biotechnol. Biochem., 76, 1239-1241.
    View publication on PubMed
Search for more literature for 3.7.1.24

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect on enzyme activity by 2,2-dipyridine, 8-hydroxyquinoline, 1,1-phenanthroline, tiron, and EDTA Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene phlG, from locus phlHGFACBDEI, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli strain XL-1 Blue Pseudomonas sp.

General Stability

General Stability Organism
2-mercaptoethanol or DTT is required for stability, the purified enzyme is rapidly inactivates without stabilizing compounds, the enzyme can be partially reactivated by 10 mM 2-mercaptoethanol to regain 20% of its initial activity, the enzyme is irreversibly inactivated by freezing and thawing Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ slight inhibition Pseudomonas sp.
diethyldicarbonate 60% inhibition at 0.5 mM Pseudomonas sp.
monoacetylphloroglucinol competitive inhibition Pseudomonas sp.
additional information no inhibition by 2-hydroxyacetophenone and 4-hydroxyacetophenone. No effect on enzyme activity by 2,2-dipyridine, 8-hydroxyquinoline, 1,1-phenanthroline, tiron, and EDTA Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 2,4-diacetylphloroglucinol recombinant enzyme, pH 7.0, 25°C Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ specifically and significantly activates the enzyme, shows a hyperbolic saturation curve in the micromolar range, binding to the enzyme might induce a conformational change that increases the catalytic effect of Zn2+ or might forma a distinct catalytic site besides that for Zn2+ Pseudomonas sp.
additional information the enzyme has a 168HXHXD172 metal binding motif. Poor effects on enzyme activity by Ca2+, Ni2+, Fe3+, Zn2+, and Mg2+ Pseudomonas sp.
Zn2+ a zinc hydrolase, located at the bottom of a deep narrow pocket of the enzyme molecule, coordinated by H129, E160, H270, and E274 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, recombinant enzyme, SDS-PAGE Pseudomonas sp.
65000
-
 recombinant enzyme, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-diacetylphloroglucinol + H2O Pseudomonas sp.
-
 2-acetylphloroglucinol + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. F7J5X9 gene phlG
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 22fold from Escherichia coli strain XL-1 Blue to homogeneity by ammonium sulfate fractionation, gel filtration, two steps of anion exchange chromatography, and hydrophobic interaction chromatograpy Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.1
-
 purified recombinant enzyme, pH 7.0, 25°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-diacetylphloroglucinol + H2O
-
 Pseudomonas sp. 2-acetylphloroglucinol + acetate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 35000, recombinant enzyme, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
Phlg
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47 55 purified recombinant enzyme, 50% activity remaining at 47°C, inactivation at 55°C Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.8
-
 2,4-diacetylphloroglucinol recombinant enzyme, pH 7.0, 25°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.087
-
 monoacetylphloroglucinol recombinant enzyme, pH 7.0, 25°C Pseudomonas sp.

General Information

General Information Comment Organism
physiological function 2,4-diacetylphloroglucinol is produced by Pseudomonas fluorescens an antibiotic that represses the growth of pathogenic microorganisms in the rhizosphere of host plants, the diacetylphloroglucinol hydrolase is a key compound in the biocontrol of Pseudomonas fluorescens Pseudomonas sp.