Cloned (Comment) | Organism |
---|---|
expression as glutathione S-transferase fusion protein | Mus musculus |
expression of C-terminally His-tagged enzyme in a bacterial expression system | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
inhibitor complexed enzyme, 3 mg/ml purified hanging drop vapor diffusion method, room temperature, in a 2:1 ratio with precipitant solution, containing 17-18% PEG 8000, 0.03-0.15 nickel acetate, no to 0.24 M sodium acetate, 0.1 M sodium cacodylate, pH 6.5, soaking in inhibitor 4-(hydroxymethylphosphinoyl)-3-oxo-butanoic acid solution for 16 h prior to data collection via X-ray diffraction, structure determination and analysis at 1.3 A resolution | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(hydroxymethylphosphinoyl)-3-oxo-butanoic acid | i.e. HMPOBA, competitive | Homo sapiens | |
4-(hydroxymethylphosphinoyl)-3-oxo-butanoic acid | i.e. HMPOBA, competitive | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens | |
Ca2+ | bound to the free enzyme | Mus musculus | |
Mg2+ | bound to the active site of the enzyme | Mus musculus | |
Mg2+ | bound to the active site of the enzyme | Homo sapiens | |
additional information | metal ligand binding distance analysis from crystal structure | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-fumarylacetoacetate + H2O | Mus musculus | final step of Phe and Tyr degradation, essential metabolic function, loss of enzyme function causes the fatal metabolic disease hereditary tyrosinemia type I, i.e. HT1 | acetoacetate + fumarate | - |
? | |
4-fumarylacetoacetate + H2O | Homo sapiens | final step of Phe and Tyr degradation, essential metabolic function, loss of enzyme function causes the fatal metabolic disease hereditary tyrosinemia type I, i.e. HT1 | acetoacetate + fumarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme | Homo sapiens |
recombinant His-tagged enzyme, fusion protein is cleaved from the fusion tag by bovine thrombin | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-fumarylacetoacetate + H2O = acetoacetate + fumarate | also acts on other 3,5- and 2,4-dioxo acids, mechanism involves a catalytic metal ion, a Glu/His catalyic diad, and a charged oxyanion hole | Homo sapiens | |
4-fumarylacetoacetate + H2O = acetoacetate + fumarate | also acts on other 3,5- and 2,4-dioxo acids, mechanism involves a catalytic metal ion, a Glu/His catalyic diad, and a charged oxyanion hole, side chain mobility in the active site | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-fumarylacetoacetate + H2O | - |
Homo sapiens | acetoacetate + fumarate | - |
? | |
4-fumarylacetoacetate + H2O | final step of Phe and Tyr degradation, essential metabolic function, loss of enzyme function causes the fatal metabolic disease hereditary tyrosinemia type I, i.e. HT1 | Mus musculus | acetoacetate + fumarate | - |
? | |
4-fumarylacetoacetate + H2O | final step of Phe and Tyr degradation, essential metabolic function, loss of enzyme function causes the fatal metabolic disease hereditary tyrosinemia type I, i.e. HT1 | Homo sapiens | acetoacetate + fumarate | - |
? | |
4-fumarylacetoacetate + H2O | formation of a tetrahedral alkoxy transition state intermediate | Mus musculus | acetoacetate + fumarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-diketonase | - |
Mus musculus |
beta-diketonase | - |
Homo sapiens |
FAH | - |
Mus musculus |
FAH | - |
Homo sapiens |
fumarylacetoacetate hydrolase | - |
Mus musculus |
fumarylacetoacetate hydrolase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
4-(hydroxymethylphosphinoyl)-3-oxo-butanoic acid | 37°C, recombinant enzyme | Homo sapiens |