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Literature summary for 3.7.1.17 extracted from

  • Lack, N.; Lowe, E.; Liu, J.; Eltis, L.; Noble, M.; Sim, E.; Westwood, I.
    Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis (2007), Acta Crystallogr. Sect. F, 64, 2-7.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.7.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pseudomonas putida strain KT4224 Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 30% (w/v) PEG 3000, 0.1?M CHES, pH 9.5 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O Mycobacterium tuberculosis high specificity ?
-
 ?
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O Mycobacterium tuberculosis H37Rv high specificity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni affinity column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O high specificity Mycobacterium tuberculosis ?
-
 ?
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O high specificity Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

Subunits Comment Organism
tetramer x-ray crystallography Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
HsaD
-
 Mycobacterium tuberculosis