Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas resinovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00251 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | pH 7.5, 25°C | Pseudomonas resinovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000, SDS-PAGe, 2 * 31300, calculated | Pseudomonas resinovorans |
31300 | - |
2 * 31000, SDS-PAGe, 2 * 31300, calculated | Pseudomonas resinovorans |
70000 | - |
gel filtration | Pseudomonas resinovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas resinovorans | Q9AQM4 | - |
- |
Pseudomonas resinovorans CA10 | Q9AQM4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas resinovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.84 | - |
pH 7.5, 25°C | Pseudomonas resinovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate + H2O | - |
Pseudomonas resinovorans | anthranilate + 2-hydroxypenta-2,4-dienoate | - |
? | |
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate + H2O | - |
Pseudomonas resinovorans CA10 | anthranilate + 2-hydroxypenta-2,4-dienoate | - |
? | |
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)hexa-2,4-dienoate + H2O | - |
Pseudomonas resinovorans | ? | - |
? | |
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)hexa-2,4-dienoate + H2O | - |
Pseudomonas resinovorans CA10 | ? | - |
? | |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
Pseudomonas resinovorans | ? | - |
? | |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
Pseudomonas resinovorans CA10 | ? | - |
? | |
additional information | hydrolytic activity decreases in the substrate order of 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate, 2-hydroxy-6-oxo-6-(2'-hydroxyphenyl)hexa-2,4-dienoate. Poor substrates: 2-hydroxy-6-oxohepta-2,4-dienoic acid, 2-hydroxymuconic semialdehyde | Pseudomonas resinovorans | ? | - |
? | |
additional information | hydrolytic activity decreases in the substrate order of 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate, 2-hydroxy-6-oxo-6-(2'-hydroxyphenyl)hexa-2,4-dienoate. Poor substrates: 2-hydroxy-6-oxohepta-2,4-dienoic acid, 2-hydroxymuconic semialdehyde | Pseudomonas resinovorans CA10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31000, SDS-PAGe, 2 * 31300, calculated | Pseudomonas resinovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, 75% residual activity | Pseudomonas resinovorans |
65 | - |
10 min, inactivation | Pseudomonas resinovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | pH 7.5, 25°C | Pseudomonas resinovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Pseudomonas resinovorans |