BRENDA - Enzyme Database show
show all sequences of 3.7.1.1

Functional characterization of the oxaloacetase encoding gene and elimination of oxalate formation in the beta-lactam producer Penicillium chrysogenum

Gombert, A.K.; Veiga, T.; Puig-Martinez, M.; Lamboo, F.; Nijland, J.G.; Driessen, A.J.; Pronk, J.T.; Daran, J.M.; Fungal Genet. Biol. 48, 831-839 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene Pc22g24830, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, functional expression in Saccharomyces cerevisiae strain IME043, IME046, and IME047
Penicillium chrysogenum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Penicillium chrysogenum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oxaloacetate + H2O
Penicillium chrysogenum
-
oxalate + acetate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
no activity in Saccharomyces cerevisiae
-
-
-
Penicillium chrysogenum
-
gene Pc22g24830
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
oxaloacetate + H2O
-
719546
Penicillium chrysogenum
oxalate + acetate
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Penicillium chrysogenum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Penicillium chrysogenum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene Pc22g24830, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, functional expression in Saccharomyces cerevisiae strain IME043, IME046, and IME047
Penicillium chrysogenum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Penicillium chrysogenum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oxaloacetate + H2O
Penicillium chrysogenum
-
oxalate + acetate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
oxaloacetate + H2O
-
719546
Penicillium chrysogenum
oxalate + acetate
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Penicillium chrysogenum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Penicillium chrysogenum
General Information
General Information
Commentary
Organism
malfunction
deletion of gene Pc22g28430 in Penicillium chrysogenum leads to complete elimination of oxalate production, whilst improving yields of the cephalosporin precursor adipoyl-6-aminopenicillinic acid
Penicillium chrysogenum
physiological function
oxaloacetate hydrolase is generally responsible for oxalate production in filamentous fungi
Penicillium chrysogenum
General Information (protein specific)
General Information
Commentary
Organism
malfunction
deletion of gene Pc22g28430 in Penicillium chrysogenum leads to complete elimination of oxalate production, whilst improving yields of the cephalosporin precursor adipoyl-6-aminopenicillinic acid
Penicillium chrysogenum
physiological function
oxaloacetate hydrolase is generally responsible for oxalate production in filamentous fungi
Penicillium chrysogenum
Other publictions for EC 3.7.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734735
Liang
Oxaloacetate acetylhydrolase g ...
Sclerotinia sclerotiorum 1980, Sclerotinia sclerotiorum
Mol. Plant Pathol.
16
559-571
2015
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1
-
1
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-
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2
-
5
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1
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2
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1
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1
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2
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
734415
Kobayashi
Oxalic acid production by citr ...
Aspergillus niger, Aspergillus niger CBS 513.88, Aspergillus niger WU-2223L
J. Ind. Microbiol. Biotechnol.
41
749-756
2014
-
-
1
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1
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2
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8
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2
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-
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-
-
-
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-
735295
Hisamori
-
Cloning and expression analysi ...
Fomitopsis palustris
Sustainable Humanosphere
9
57-64
2013
-
-
1
-
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-
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
-
1
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-
-
1
-
-
-
-
1
1
-
-
-
719546
Gombert
Functional characterization of ...
no activity in Saccharomyces cerevisiae, Penicillium chrysogenum
Fungal Genet. Biol.
48
831-839
2011
-
-
1
-
-
-
-
-
-
1
-
1
-
6
-
-
-
-
-
-
-
-
1
-
1
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-
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1
-
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1
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-
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1
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1
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-
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1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
719879
Chen
Structure of oxalacetate acety ...
Cryphonectria parasitica
J. Biol. Chem.
285
26685-26696
2010
-
-
-
1
-
-
1
4
-
3
-
1
-
3
-
-
-
-
-
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3
2
1
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3
1
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1
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1
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4
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3
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1
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3
2
1
-
-
3
1
-
-
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-
3
3
-
-
-
693684
Narayanan
Structure and function of 2,3- ...
Aspergillus niger
J. Mol. Biol.
386
486-503
2009
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1
1
6
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4
6
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1
4
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1
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1
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1
1
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7
1
1
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3
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1
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1
6
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4
3
6
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1
4
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1
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1
1
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7
1
1
-
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1
1
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5
5
686268
do Rio
Production of Calcium Oxalate ...
Moniliophthora perniciosa FA553
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56
363-370
2008
-
1
-
-
-
-
-
-
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1
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6
-
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1
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1
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1
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1
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1
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1
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689936
Joosten
Identification of fungal oxalo ...
Botrytis cinerea, Dianthus caryophyllus
Proteins
70
157-166
2008
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2
-
-
-
-
18
1
-
-
1
-
6
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2
-
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3
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2
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18
2
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2
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18
1
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1
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2
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-
3
-
2
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-
18
2
-
-
-
-
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-
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-
-
687682
Han
Oxaloacetate hydrolase, the C- ...
Aspergillus niger, Botrytis cinerea
J. Biol. Chem.
282
9581-9590
2007
-
2
2
-
-
-
2
4
-
-
3
2
-
6
-
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1
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4
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2
1
1
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9
1
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1
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2
2
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2
1
4
-
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3
2
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1
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4
-
2
1
1
-
-
9
1
-
-
-
-
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-
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-
668669
Sexton
Comparison of transcription of ...
Sclerotinia sclerotiorum
FEMS Microbiol. Lett.
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150-160
2006
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6
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1
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670169
Sakai
Subcellular localization of gl ...
Fomitopsis palustris
Microbiology
152
1857-1866
2006
-
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1
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1
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3
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1
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1
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668652
Yoon
A metabolic role of the glyoxy ...
Fomitopsis palustris
FEMS Microbiol. Lett.
217
9-14
2002
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1
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670718
Munir
A physiological role for oxali ...
Fomitopsis palustris
Proc. Natl. Acad. Sci. USA
98
11126-11130
2001
-
-
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2
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1
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1
1
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210608
Pedersen
Cloning and characterization o ...
Aspergillus niger
Mol. Gen. Genet.
263
281-286
2000
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1
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1
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2
1
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2
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1
1
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1
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1
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210609
Ruijter
-
Oxalic acid production by Aspe ...
Aspergillus niger
Microbiology
145
2569-2576
1999
-
-
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1
1
1
1
1
1
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1
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1
1
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210610
Houck
Oxalic acid biosynthesis and o ...
Cryphonectria parasitica, Streptomyces cattleya
Arch. Biochem. Biophys.
259
58-65
1987
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1
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4
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2
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1
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2
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2
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1
1
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210611
Muller
Utilization of gluconate by As ...
Aspergillus niger
Zentralbl. Mikrobiol.
141
461-469
1986
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210612
Lenz
Partial purification and some ...
Aspergillus niger
Eur. J. Biochem.
65
225-236
1976
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210613
Muller
Oxalate accumulation from citr ...
Aspergillus niger
Arch. Microbiol.
103
185-189
1975
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210614
Maxwell
-
Oxalate formation in Whetzelin ...
Sclerotinia sclerotiorum
Physiol. Plant Pathol.
3
279-288
1973
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210615
Hayaishi
-
Enzymatic formation of oxalate ...
Aspergillus niger
J. Am. Chem. Soc.
78
5126-5127
1956
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