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Literature summary for 3.7.1.1 extracted from

  • Gombert, A.K.; Veiga, T.; Puig-Martinez, M.; Lamboo, F.; Nijland, J.G.; Driessen, A.J.; Pronk, J.T.; Daran, J.M.
    Functional characterization of the oxaloacetase encoding gene and elimination of oxalate formation in the beta-lactam producer Penicillium chrysogenum (2011), Fungal Genet. Biol., 48, 831-839.
    View publication on PubMed
Search for more literature for 3.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene Pc22g24830, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, functional expression in Saccharomyces cerevisiae strain IME043, IME046, and IME047 Penicillium chrysogenum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + H2O Penicillium chrysogenum
-
 oxalate + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
no activity in Saccharomyces cerevisiae
-
-
-
Penicillium chrysogenum
-
 gene Pc22g24830
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + H2O
-
 Penicillium chrysogenum oxalate + acetate
-
 ?

Synonyms

Synonyms Comment Organism
OAH
-
 Penicillium chrysogenum
oxaloacetate hydrolase
-
 Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Penicillium chrysogenum

General Information

General Information Comment Organism
malfunction deletion of gene Pc22g28430 in Penicillium chrysogenum leads to complete elimination of oxalate production, whilst improving yields of the cephalosporin precursor adipoyl-6-aminopenicillinic acid Penicillium chrysogenum
physiological function oxaloacetate hydrolase is generally responsible for oxalate production in filamentous fungi Penicillium chrysogenum