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Literature summary for 3.7.1.1 extracted from

  • Joosten, H.J.; Han, Y.; Niu, W.; Vervoort, J.; Dunaway-Mariano, D.; Schaap, P.J.
    Identification of fungal oxaloacetate hydrolyase within the isocitrate lyase/PEP mutase enzyme superfamily using a sequence marker-based method (2008), Proteins, 70, 157-166.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET-3c Dianthus caryophyllus
into the vector pET-3c Botrytis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
(2R,3S)-2,3-dimethylmalate mutant S260P Botrytis cinerea
0.054
-
(2R,3S)-2,3-dimethylmalate mutant S260A Botrytis cinerea
0.065
-
oxaloacetate wild-type protein Botrytis cinerea
0.13
-
oxaloacetate wild-type protein Dianthus caryophyllus
0.14
-
(2R,3S)-2,3-dimethylmalate wild-type protein Botrytis cinerea
0.15
-
oxaloacetate mutant S260P, trial 1 Botrytis cinerea
0.22
-
oxaloacetate mutant S260P, trial 2 Botrytis cinerea
0.53
-
(2R,3S)-2-ethyl-3-methylmalate wild-type protein Dianthus caryophyllus
0.56
-
(2R,3S)-2-ethyl-3-methylmalate mutant S257A Dianthus caryophyllus
0.66
-
(2R,3S)-2-ethyl-3-methylmalate mutant S257P Dianthus caryophyllus
0.7
-
oxaloacetate mutant S260A, trial 1 Botrytis cinerea
0.84
-
oxaloacetate mutant S257P Dianthus caryophyllus
1.307
-
oxaloacetate mutant S260A, trial 2 Botrytis cinerea
1.53
-
oxaloacetate mutant S257A Dianthus caryophyllus
2.6
-
oxaloacetate mutant S257T, trial 2 Dianthus caryophyllus
3.6
-
oxaloacetate mutant S257T, trial 1 Dianthus caryophyllus
7
-
oxaloacetate mutant S260T, trial 1 Botrytis cinerea
7.7
-
oxaloacetate mutant S260T, trial 2 Botrytis cinerea

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-2,3-dimethylmalate Botrytis cinerea
-
pyruvate + propionic acid
-
?

Organism

Organism UniProt Comment Textmining
Botrytis cinerea Q6PNM8
-
-
Dianthus caryophyllus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
proteins are purified to homogeneity Dianthus caryophyllus
proteins are purified to homogeneity Botrytis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-2,3-dimethylmalate
-
Botrytis cinerea pyruvate + propionic acid
-
?
(2R,3S)-2-ethyl-3-methylmalate + H2O
-
Dianthus caryophyllus ?
-
?
oxaloacetate + H2O
-
Dianthus caryophyllus oxalate + acetate
-
?
oxaloacetate + H2O
-
Botrytis cinerea oxalate + acetate
-
?

Synonyms

Synonyms Comment Organism
OAH
-
Botrytis cinerea
OAH-active petal death protein
-
Dianthus caryophyllus
oxaloacetase
-
Botrytis cinerea
oxaloacetate acetylhydrolase
-
Botrytis cinerea
oxaloacetate hydrolase
-
Botrytis cinerea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
activity assay Dianthus caryophyllus
25
-
activity assay Botrytis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0249
-
(2R,3S)-2,3-dimethylmalate mutant S260A Botrytis cinerea
0.0293
-
(2R,3S)-2,3-dimethylmalate wild-type protein Botrytis cinerea
0.074
-
(2R,3S)-2,3-dimethylmalate mutant S260P Botrytis cinerea
0.71
-
oxaloacetate mutant S260A, trial 2 Botrytis cinerea
0.83
-
oxaloacetate mutant S260A, trial 1 Botrytis cinerea
0.96
-
oxaloacetate mutant S260P, trial 1 Botrytis cinerea
0.98
-
oxaloacetate mutant S260P, trial 2 Botrytis cinerea
1.14
-
oxaloacetate mutant S257T, trial 1 Dianthus caryophyllus
1.16
-
oxaloacetate mutant S257T, trial 2 Dianthus caryophyllus
2.59
-
oxaloacetate mutant S260T, trial 1 Botrytis cinerea
2.72
-
oxaloacetate wild-type protein Dianthus caryophyllus
2.91
-
oxaloacetate mutant S257A Dianthus caryophyllus
3.6
-
oxaloacetate mutant S260T, trial 2 Botrytis cinerea
4.1
-
oxaloacetate mutant S257P Dianthus caryophyllus
5.4
-
(2R,3S)-2-ethyl-3-methylmalate mutant S257A Dianthus caryophyllus
8.3
-
(2R,3S)-2-ethyl-3-methylmalate mutant S257P Dianthus caryophyllus
8.4
-
(2R,3S)-2-ethyl-3-methylmalate wild-type protein Dianthus caryophyllus
17.4
-
oxaloacetate wild-type protein Botrytis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
activity assay Dianthus caryophyllus
7.5
-
activity assay Botrytis cinerea