Literature summary for 3.6.5.6 extracted from

Dougherty, C.A.; Sage, C.R.; Davis, A.; Farrell, K.W.
Mutation in the beta-tubulin signature motif suppresses microtubule GTPase activity and dynamics, and slows mitosis (2001), Biochemistry, 40, 15725-15732.

Search for more literature for 3.6.5.6

Protein Variants

Protein Variants	Comment	Organism
T143G	mutation in tubulin signature motif of beta-tubulin, both GTP-binding affinity and microtubule-dependent GTPase activity are reduced at least 15 fold, mutant cells have a delay in mitosis	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	beta-tubulin	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Saccharomyces cerevisiae	GDP + phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
100	-	GTP	pH 6.8, 30°C, mutant T143G	Saccharomyces cerevisiae
1500	-	GTP	pH 6.8, 30°C, wild-type	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)