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Literature summary for 3.6.5.6 extracted from
- The colchicine-induced GTPase activity of tubulin: State of the product. Activation by microtubule-promoting cosolvents (1994), Biochemistry, 33, 6253-6261.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,3,4-trimethoxy-4'-(carbomethoxy)-1,1'-biphenyl | initiation of the GTPase | Bos taurus |  |
| allocolchicine | - |
Bos taurus | |
| Colchicine | induces GTPase activity | Bos taurus | |
| Colchicine | induced by all colchicine analogues that contain rings A, C or C' | Bos taurus | |
| dimethyl sulfoxide | cosolvent stabilizes tubulin | Bos taurus | |
| glycerol | cosolvent stabilizes tubulin, GTP hydrolysis increases by addition of 3.4 M | Bos taurus | |
| sodium glutamate | cosolvent stabilizes tubulin | Bos taurus | |
| sucrose | cosolvent stabilizes tubulin | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
GTP | induced by allocolchicine | Bos taurus | |
| 0.009 | - |
GTP | induced by colchicine | Bos taurus | |
| 0.01 | - |
GTP | induced by 2,3,4-trimethoxy-4'-(carbomethoxy)-1,1'-biphenyl | Bos taurus | |
| 0.011 | - |
GTP | induced by 2,3,4-trimethoxy-4'-acetyl-1,1'-biphenyl | Bos taurus | |
| 0.012 | - |
GTP | induced by 2-methoxy-5-(2,3,4-trimethoxyphenyl)-2,4,6-cycloheptatrien-1-one | Bos taurus | |
| 0.015 | - |
GTP | induced by 2,3,4,4-tetramethoxy-1,1'-biphenyl | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity, strong increase which reaches a plateau at 4 mM MgCl2 | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
gel filtration in presence of glycerol | Bos taurus |
| 220000 | - |
gel filtration in absence of glycerol | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Bos taurus | - |
GDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | 20 | - |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Bos taurus | GDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | composed of 2 similar, but not identical chains designated alpha and beta | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
GTP | in 0.01 M sodium phosphate and 4 mM MgCl2, pH 7 | Bos taurus | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7.4 | tubulin stable in this range | Bos taurus |
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