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Literature summary for 3.6.5.5 extracted from
- Enhancement of dynamin polymerization and GTPase activity by Arc/Arg3.1 (2015), Biochim. Biophys. Acta, 1850, 1310-1318 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Arc/Arg3.1 | enhances dynamin polymerization and GTPase activity. The activity-regulated cytoskeleton-associated protein, Arc, is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Bacterially-expressed His6-Arc facilitates the polymerization of Dyn2 and stimulates its GTPase activity under physiologic conditions (37°C and 100 mM NaCl). At lower ionic strength Arc also stabilizes preformed Dyn2 polymers against GTP-dependent disassembly, thereby prolonging assembly-dependent GTP hydrolysis catalyzed by Dyn2. Arc also increases the GTPase activity of Dyn3, an isoform of implicated in dendrite remodeling, but does not affect the activity of Dyn1, a neuron-specific isoform involved in synaptic vesicle recycling. Analysis of recombinant full-length mouse Arc and of His6-tagged fragments, including Arc-N (residues 1-227), Arc-C (residues 228-396), and Arc-(DELTA195-214). While residues 195-214 may contribute to dynamin binding, additional binding determinants are likely to be present in the N-terminal portion of Arc. Arc stimulates the assembly-dependent GTPase activity of isozymes Dyn2 and Dyn3. The effect of Arc on GTPase activity of dynamin mirrors its effect on dynamin assembly as measured by turbidity | Rattus norvegicus | |
| Arc/Arg3.1 | enhances dynamin polymerization and GTPase activity. The activity-regulated cytoskeleton-associated protein, Arc, is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Bacterially-expressed His6-Arc increases the GTPase activity of Dyn3, an isoform of implicated in dendrite remodeling. Analysis of recombinant full-length mouse Arc and of His6-tagged fragments, including Arc-N (residues 1-227), Arc-C (residues 228-396), and Arc-(DELTA195-214). Arc stimulates the assembly-dependent GTPase activity of isozymes Dyn2 and Dyn3. The effect of Arc on GTPase activity of dynamin mirrors its effect on dynamin assembly as measured by turbidity | Rattus norvegicus | |
| additional information | activity-regulated cytoskeleton-associated protein, Arc (Arc/Arg3.1) is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Bacterially-expressed His6-Arc does not affect the activity of Dyn1, a neuron-specific isoform involved in synaptic vesicle recycling, in contrast to the other Dyn isozymes, Arc stimulates the assembly-dependent GTPase activity of Dyn2 and Dyn3. Analysis of recombinant full-length mouse Arc and of His6-tagged fragments, including Arc-N (residues 1-227), Arc-C (residues 228-396), and Arc-(DELTA195-214) | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Dnm1, recombinant expression of C-terminally His6-tagged isozyme Dyn1 in Spodoptera frugiperda Sf9 cells | Rattus norvegicus |
| gene Dnm2, recombinant expression of C-terminally His6-tagged isozyme Dyn2 in Spodoptera frugiperda Sf9 cells | Rattus norvegicus |
| gene Dnm3, recombinant expression of C-terminally His6-tagged isozyme Dyn3 in Spodoptera frugiperda Sf9 cells | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Arc/Arg3.1 | enhances dynamin polymerization and GTPase activity. The activity-regulated cytoskeleton-associated protein, Arc, is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Bacterially-expressed His6-Arc facilitates the polymerization of Dyn2 and stimulates its GTPase activity under physiologic conditions (37°C and 100 mM NaCl). At lower ionic strength Arc also stabilizes preformed Dyn2 polymers against GTP-dependent disassembly, thereby prolonging assembly-dependent GTP hydrolysis catalyzed by Dyn2. Analysis of recombinant full-length mouse Arc and of His6-tagged fragments, including Arc-N (residues 1-227), Arc-C (residues 228-396), and Arc-(DELTA195-214). At physiologic ionic strength, Arc facilitates Dyn2 self-assembly but does not prevent GTP-dependent Dyn2 disassembly, which is expected to suppress rather than activate endocytosis | Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Rattus norvegicus | - |
GDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P21575 | - |
- |
| Rattus norvegicus | P39052 | - |
- |
| Rattus norvegicus | Q08877 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged isozyme Dyn1 from Sf9 cells by nickel affinity chromatography and dialysis | Rattus norvegicus |
| recombinant C-terminally His6-tagged isozyme Dyn2 from Sf9 cells by nickel affinity chromatography and dialysis | Rattus norvegicus |
| recombinant C-terminally His6-tagged isozyme Dyn3 from Sf9 cells by nickel affinity chromatography and dialysis | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| neuron | Dyn1 is a neuron-specific isoform | Rattus norvegicus | - |
| testis | Dyn3 is a testicular isozyme | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Rattus norvegicus | GDP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dyn1 | - |
Rattus norvegicus |
| DYN2 | - |
Rattus norvegicus |
| Dyn3 | - |
Rattus norvegicus |
| dynamin | - |
Rattus norvegicus |
| dynamin 1aa | - |
Rattus norvegicus |
| dynamin 2aa | - |
Rattus norvegicus |
| dynamin 3aa | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Dyn1 is a neuron-specific isoform involved in synaptic vesicle recycling | Rattus norvegicus |
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