Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged or FLAG-tagged wild-type and mutant enzymes in HeLa cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
R386G | site-directed mutagenesis, replacement of Arg386 with Gly in dynamin 1 middle domain reduces GTPase activity and oligomer stability in the absence of lipids, while in presence of phosphatidylserine liposomes, the intermolecular interactions of dynamin 1 are not affected. The mutant is a monomer with reduced GTPase activity compared to the wild-type enzyme | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Mus musculus | - |
GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P39053 | gene Dyn1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Mus musculus | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme's middle domain is involved in the formation of functional oligomers. Wild-type enzyme forms oligomers, while enzyme mutant R386G prefers to form monomers | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
Dyn1 | - |
Mus musculus |
dynamin 1 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | R386G mutation in dynamin 1 middle domain reduces GTPase activity and oligomer stability in the absence of lipids. Vesicle formed in endocytosis are smaller in enzyme mutant R386G cells compared to wild-type | Mus musculus |
physiological function | dynamin plays an important role in membrane fission during endocytosis. The dynammin GTPase is important for dynamin oligomer complex dissociation in absence of lipids | Mus musculus |