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Literature summary for 3.6.5.5 extracted from

  • Anantharam, A.; Bittner, M.A.; Aikman, R.L.; Stuenkel, E.L.; Schmid, S.L.; Axelrod, D.; Holz, R.W.
    A new role for the dynamin GTPase in the regulation of fusion pore expansion (2011), Mol. Biol. Cell, 22, 1907-1918.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
Dyn1 expression in bovine adrenal chromaffin cells, coexpression with granule marker VMAT2-pH, the amount of dynamin on the plasma membrane in dynamin-transfected cells was increased 2.5 to 3.5 times compared with endogenous dynamin in nontransfected cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
T141A the Dyn1mutant shows increased GTPase activity and GTP affinity, the frequency of endocytosis after fusion events is increased to 22% compared to 17% in cells not expressing the recombinant protein, the mutant enhances transferrin internalization Saccharomyces cerevisiae
T65A the Dyn1mutant shows greatly reduced GTPase activity and reduced GTP affinity, the frequency of endocytosis after fusion events is decreased to 7% compared to 17% in cells not expressing the recombinant protein, the mutant reuces transferrin internalization Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Saccharomyces cerevisiae 5886
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Synonyms

Synonyms Comment Organism
Dyn-1
-
 Saccharomyces cerevisiae
dynamin-1
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction a dynamin-1 mutant with in­creased GTPase activity results in transient deformations consistent with rapid fusion pore widening after exocytosis, while a Dyn1 mutant with decreased activity slows fusion pore widen­ing by stabilizing postfusion granule membrane deformations Saccharomyces cerevisiae
additional information effects of transiently expressed Dyn1WT on membrane topological changes following granule fusion in bovine adrenal chromaffin cells, overview. Expression of dynamin GTPase mutants altered the speed of NPY-Cer release Saccharomyces cerevisiae
physiological function dynamin is a master regulator of membrane fission in endocytosis. In addition to its role in endocytosis, GTPase activity of dynamin regulates the rapidity of fusion pore expansion from tens of milliseconds to seconds after fusion. Membrane-sculpting repertoire of dynamin to include the regulation of immediate post­fusion events in exocytosis that control the rate of release of soluble granule contents Saccharomyces cerevisiae