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Literature summary for 3.6.5.5 extracted from

  • Anwar, K.; Klemm, R.W.; Condon, A.; Severin, K.N.; Zhang, M.; Ghirlando, R.; Hu, J.; Rapoport, T.A.; Prinz, W.A.
    The dynamin-like GTPase Sey1p mediates homotypic ER fusion in S. cerevisiae (2012), J. Cell Biol., 197, 209-217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.5

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged wild-type and mutant GST-SEY1 in Escherichia coli strain BL21 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A592V the mutant acts similar to the wild-type enzyme Saccharomyces cerevisiae
K50A the mutant shows reduced GTPase activity compared to the wild-type and is unable to mediate proteoliposomal fusion Saccharomyces cerevisiae
additional information construction of mutant Sey1-DELTATM by replacement of the transmembrane domains of Sey1, amino acids 681-727, with a 12-amino acid linker, the mutant rans as a monomer in the absence of nucleotide or in the presence of GDP but as a dimer in the presence of GDP and AlFx as a mimic of the transition state of nucleotide hydrolysis Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Saccharomyces cerevisiae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Saccharomyces cerevisiae
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 gene SEY1, strains RSY275, ACY85, WPY804, and WPY805
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant GST-SEY1 from Escherichia coli strain BL21 by glutathione affinity chromatography, the tag is cleaved off by thrombin Saccharomyces cerevisiae

Renatured (Commentary)

Renatured (Comment) Organism
purified recombinant enzyme at 1 mg/ml is reconstituted in liposomes Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Saccharomyces cerevisiae GDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer Sey1p undergoes GTP-dependent dimerization Saccharomyces cerevisiae
More Sey1p dimerizes in the presence of GDP AlFx Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
dynamin-like GTPase
-
 Saccharomyces cerevisiae
dynamin-like guanosine triphosphatase
-
 Saccharomyces cerevisiae
Sey1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction the absence of Sey1p results in the endoplasmic reticulum undergoing delayed fusion in vivo and proteoliposomes containing purified Sey1p fused in a GTP-dependent manner in vitro, phenotypes, overview Saccharomyces cerevisiae
additional information human ATL1 can functionally replace Sey1p in yeast Saccharomyces cerevisiae
physiological function Seyp1 mediates homotypic membrane fusion required to maintain endoplasmic reticulum morphology, overview Saccharomyces cerevisiae