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Literature summary for 3.6.5.5 extracted from

  • Sever, S.; Skoch, J.; Newmyer, S.; Ramachandran, R.; Ko, D.; McKee, M.; Bouley, R.; Ausiello, D.; Hyman, B.T.; Bacskai, B.J.
    Physical and functional connection between auxilin and dynamin during endocytosis (2006), EMBO J., 25, 4163-4174.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.5

Cloned(Commentary)

Cloned (Comment) Organism
adenovirus expressing the dynamin mutant K44A is constructed Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
I690K mutant is assembly incompetent and inactive for endocytosis Rattus norvegicus
K44A mutant of dynamin that cannot bind GTP Rattus norvegicus
K694A mutant of dynamin that increases the rate of endocytosis owing to impaired assembly into higher order structures Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 GTP I690K mutant, high salt Rattus norvegicus
0.025
-
 GTP I690K mutant, low salt Rattus norvegicus
0.025
-
 GTP K694A mutant, low salt Rattus norvegicus
0.028
-
 GTP I690K mutant, + GAP Rattus norvegicus
0.031
-
 GTP wild-type dynamin, + GAP Rattus norvegicus
0.033
-
 GTP K694A mutant, + GAP Rattus norvegicus
0.035
-
 GTP wild-type dynamin, low salt Rattus norvegicus
0.04
-
 GTP wild-type dynamin, high salt Rattus norvegicus
0.044
-
 GTP K694A mutant, high salt Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-
membrane
-
 Rattus norvegicus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Rattus norvegicus
-
 GDP + phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
IMCD cell
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Rattus norvegicus GDP + phosphate
-
 ir

Subunits

Subunits Comment Organism
dimer
-
 Rattus norvegicus
More assembly into higher order structures such as rings or spirals Rattus norvegicus
tetramer dynamin dimers need to oligomerize into tetramers for efficient GTP binding Rattus norvegicus

Synonyms

Synonyms Comment Organism
dynamin
-
 Rattus norvegicus
dynamin-2
-
 Rattus norvegicus