Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.5.4 extracted from

  • Egea, P.F.; Tsuruta, H.; de Leon, G.P.; Napetschnig, J.; Walter, P.; Stroud, R.M.
    Structures of the signal recognition particle receptor from the archaeon Pyrococcus furiosus: implications for the targeting step at the membrane (2008), PLoS ONE, 3, e3619.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)-Rosetta2 cells Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
free and GDP-magnesium-bound forms, hanging drop vapour diffusion method, the hexagonal form grows in 1.1-1.5 M ammonium phosphate and 100 mM sodium acetate pH 5.0. The monoclinic form grows in 0.9-1.2 M lithium sulfate, 0.4-0.6 M ammonium sulfate, and 100 mM sodium citrate pH 5.0. For the GDP-bound structure, best crystals grow in 14-17% (w/v) PEG 8000 and 100 mM Tris pH 8.0 Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34900
-
 apo-enzyme, analytical ultracentrifugation and small-angle X-ray scattering Pyrococcus furiosus
35810
-
 apo-enzyme, calculated from amino acid sequence Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U051
-
-

Purification (Commentary)

Purification (Comment) Organism
heat selective precipitation, cobalt-chelating affinity chromatography, gel filtration, and ion-exchange chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Pyrococcus furiosus GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
FtsY
-
 Pyrococcus furiosus
signal recognition particle receptor
-
 Pyrococcus furiosus
SRP
-
 Pyrococcus furiosus