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Literature summary for 3.6.5.4 extracted from

  • Egea, P.F.; Shan, S.O.; Napetschnig, J.; Savage, D.F.; Walter, P.; Stroud, R.M.
    Substrate twinning activates the signal recognition particle and its receptor (2004), Nature, 427, 215-221.
    View publication on PubMed
Search for more literature for 3.6.5.4

Activating Compound

Activating Compound Comment Organism Structure
signal recognition particle receptor SRP and its receptor stimulate each other’s GTPase activity, mechanism of reciprocal activation, substrate twinning activates the signal recognition particle and its receptor Thermus aquaticus

Cloned(Commentary)

Cloned (Comment) Organism
N and G domains of Ffh, expression in Escherichia coli BL21(DE3)-Rosetta Thermus aquaticus

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray structure of a complex of the N and G domains of Ffh with the GTPase FtsY of the SRP receptor in the presence of the non-hydrolyzable GTP analogue GMPPCP Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Thermus aquaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ bound Thermus aquaticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Thermus aquaticus protein targeting to the plasma membrane, signal recognition particle cycle, SRP and its receptor stimulate each other’s GTPase activity, GTP hydrolysis ensures unidirectional targeting of cargo through a translocation pore in the membrane GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O protein targeting to the plasma membrane, signal recognition particle cycle, SRP and its receptor stimulate each other’s GTPase activity, GTP hydrolysis ensures unidirectional targeting of cargo through a translocation pore in the membrane Thermus aquaticus GDP + phosphate
-
 ?
GTP + H2O complex formation of Ffh with the signal recognition particle receptor aligns the two GTP substrate molecules in a symmetrical, composite active site, and the 3’-OH groups are essential for association, reciprocal activation and catalysis, this circle of twinned interactions is severed on hydrolysis, leading to complex dissociation after cargo delivery, structure Thermus aquaticus GDP + phosphate
-
 ?
additional information not: GMPPCP Thermus aquaticus ?
-
 ?

Synonyms

Synonyms Comment Organism
Ffh N and G domains Thermus aquaticus