Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.5.3 extracted from

  • Gibbs, M.R.; Moon, K.M.; Chen, M.; Balakrishnan, R.; Foster, L.J.; Fredrick, K.
    Conserved GTPase LepA (elongation factor 4) functions in biogenesis of the 30S subunit of the 70S ribosome (2017), Proc. Natl. Acad. Sci. USA, 114, 980-985 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.3

Activating Compound

Activating Compound Comment Organism Structure
additional information the GTPase activity of LepA, like that of other translational GTPases, is stimulated by interactions with both subunits of the ribosome Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information the DELTAlepA mutation is introduced via Hfr-mediated conjugation into each strain of the Keio collection, mutation DELTAlepA confers a synthetic growth defect in the absence of RsgA, a conserved GTPase known to be involved in SSU biogenesis. Mutation DELTArsgA slows the growth of Escherichia coli substantially, increasing the doubling time by 18 min. The growth defect is rescued by plasmid pRSGA, which contains the rsgA gene and its native promoter region, confirming that loss of RsgA is responsible for the phenotype. Precursor 17S rRNA accumulates in pre-30S and 30S particles in the absence of LepA, phenotype overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P60785
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
EF-4
-
 Escherichia coli
EF4
-
 Escherichia coli
elongation factor 4
-
 Escherichia coli
LepA
-
 Escherichia coli
translational GTPase
-
 Escherichia coli

General Information

General Information Comment Organism
evolution the translational GTPase LepA is a highly conserved bacterial protein Escherichia coli
malfunction in cells lacking LepA, immature 30S particles accumulate. Four proteins are specifically underrepresented in these particles (S3, S10, S14, and S21) all of which bind late in the assembly process and contribute to the folding of the 3' domain of 16S rRNA. Processing of 16S rRNA is also delayed in the mutant strain, as indicated by increased levels of precursor 17S rRNA in assembly intermediates. Mutation DELTAlepA confers a synthetic growth phenotype in absence of RsgA, another GTPase, well known to act in 30S subunit assembly. Analysis of the DELTArsgA strain reveals accumulation of intermediates that resemble those seen in the absence of LepA. The growth defect is rescued by plasmid pRSGA, which contains the rsgA gene and its native promoter region Escherichia coli
metabolism RsgA and LepA play partially redundant roles to ensure efficient 30S assembly Escherichia coli
physiological function LepA functions in ribosome biogenesis, analysis of the role of LepA in ribosome assembly, overview. LepA functions in biogenesis of the 30S subunit of the ribosome, rather than in translation elongation. The GTPase activity of LepA is stimulated by interactions with both subunits of the ribosome, implying that LepA acts at a late stage of assembly, in the context of the 70S ribosome Escherichia coli