Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.5.3 extracted from

  • Masullo, M.; Raimo, G.; Parente, A.; Gambacorta, A.; De Rosa, M.; Bocchini, V.
    Properties of the elongation factor 1 alpha in the thermoacidophilic archaebacterium Sulfolobus solfataricus (1991), Eur. J. Biochem., 199, 529-537.
    View publication on PubMed
Search for more literature for 3.6.5.3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
-20°C, stable for several months Saccharolobus solfataricus

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
aEF-1alpha
-
 Saccharolobus solfataricus
elongation factor 1 alpha
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 the rate of nucleotide binding to aEF-1 a increased with temperature, reaching a maximum at 95°C Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 after a 6 h treatment at 80°C and 87°C, the residual activity is 80% and 54%, respectively; at 95°C the [3H]GDP-binding activity became one half of the initial activity after about 30 min. After 80 days at 25°C, a crude aEF-la preparation retained 50% of the maximum nucleotide-binding capacity Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoelectric focusing, pH-gradient 3-10
-
 9.1