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Literature summary for 3.6.5.3 extracted from
- The site for GTP hydrolysis on the archaeal elongation factor 2 is unmasked by aliphatic alcohols (1996), Biochimie, 78, 832-837.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1-propanol | stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol | Saccharolobus solfataricus |  |
| 2-propanol | stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol | Saccharolobus solfataricus | |
| ethanol | stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol | Saccharolobus solfataricus | |
| ethylene glycoI | 60%, 300fold stimulation | Saccharolobus solfataricus | |
| glycerol | stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol | Saccharolobus solfataricus | |
| methanol | stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0009 | - |
GTP | pH 7.8, 50°C | Saccharolobus solfataricus | |
| 0.001 | - |
GTP | pH 7.8, 75°C | Saccharolobus solfataricus | |
| 0.0012 | - |
GTP | pH 7.8, 60°C | Saccharolobus solfataricus | |
| 0.0013 | - |
GTP | pH 7.8, 80°C | Saccharolobus solfataricus | |
| 0.0014 | - |
GTP | pH 7.8, 70°C | Saccharolobus solfataricus | |
| 0.0053 | - |
GTP | pH 7.8, 87°C | Saccharolobus solfataricus | |
| 0.02 | - |
GTP | pH 7.8, 91°C | Saccharolobus solfataricus | |
| 0.0532 | - |
GTP | pH 7.8, 95°C | Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | 10 mM, about 8fold stimulation | Saccharolobus solfataricus | |
| CaCl2 | 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness | Saccharolobus solfataricus | |
| CoCl2 | 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness | Saccharolobus solfataricus | |
| MgCl2 | 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness | Saccharolobus solfataricus | |
| MnCl2 | 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness | Saccharolobus solfataricus | |
| additional information | GTPase stimulated by ethylene glycol and BaCl2 does not require the presence of univalent cations. Li+, Na+, K+ or NH4+ added singularly up to 1 M concentration, do not produce any significant stimulation of SsEF-2 GTPase either in the absence or in the presence of ethylene glycol. They reduce the stimulation of SsEF-2 GTPase by ethylene glycol plus BaCl2 or SrCl2 | Saccharolobus solfataricus | |
| SrCl2 | 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness | Saccharolobus solfataricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus ATCC 49255 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Saccharolobus solfataricus | GDP + phosphate | - |
? | |
| GTP + H2O | - |
Saccharolobus solfataricus ATCC 49255 | GDP + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
GTP | pH 7.8, 50°C | Saccharolobus solfataricus | |
| 0.038 | - |
GTP | pH 7.8, 60°C | Saccharolobus solfataricus | |
| 0.118 | - |
GTP | pH 7.8, 70°C | Saccharolobus solfataricus | |
| 0.152 | - |
GTP | pH 7.8, 75°C | Saccharolobus solfataricus | |
| 0.23 | - |
GTP | pH 7.8, 80°C | Saccharolobus solfataricus | |
| 0.512 | - |
GTP | pH 7.8, 87°C | Saccharolobus solfataricus | |
| 0.558 | - |
GTP | pH 7.8, 95°C | Saccharolobus solfataricus | |
| 0.89 | - |
GTP | pH 7.8, 91°C | Saccharolobus solfataricus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
GTP | pH 7.8, 95°C | Saccharolobus solfataricus | |
| 26 | - |
GTP | pH 7.8, 50°C | Saccharolobus solfataricus | |
| 32 | - |
GTP | pH 7.8, 60°C | Saccharolobus solfataricus | |
| 45 | - |
GTP | pH 7.8, 91°C | Saccharolobus solfataricus | |
| 84 | - |
GTP | pH 7.8, 70°C | Saccharolobus solfataricus | |
| 97 | - |
GTP | pH 7.8, 87°C | Saccharolobus solfataricus | |
| 152 | - |
GTP | pH 7.8, 75°C | Saccharolobus solfataricus | |
| 177 | - |
GTP | pH 7.8, 80°C | Saccharolobus solfataricus | |
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