Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Thiostrepton | inhibits the ribosome-stimulated GTPase activity of EF-G and EF4. An EF-G mutant lacking domains 4 and 5 is insensitive to the effects of thiostrepton on both GTPase activity and ribosome binding | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Escherichia coli | - |
GDP + phosphate | - |
? | |
GTP + H2O | Escherichia coli MRE 600 | - |
GDP + phosphate | - |
? | |
additional information | Escherichia coli | EF4-ribosome interactions during reverse translocation, overview | ? | - |
? | |
additional information | Escherichia coli MRE 600 | EF4-ribosome interactions during reverse translocation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli MRE 600 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Escherichia coli | GDP + phosphate | - |
? | |
GTP + H2O | reaction using Escherichia coli 70S ribosomes, determination of binding of GTPases to 70S ribosomes in the GTP state, formation of 70S ribosome-tRNAPhe -GTPase-GDPNP complexes, multiple-turnover GTP hydrolysis | Escherichia coli | GDP + phosphate | - |
? | |
GTP + H2O | - |
Escherichia coli MRE 600 | GDP + phosphate | - |
? | |
GTP + H2O | reaction using Escherichia coli 70S ribosomes, determination of binding of GTPases to 70S ribosomes in the GTP state, formation of 70S ribosome-tRNAPhe -GTPase-GDPNP complexes, multiple-turnover GTP hydrolysis | Escherichia coli MRE 600 | GDP + phosphate | - |
? | |
additional information | EF4-ribosome interactions during reverse translocation, overview | Escherichia coli | ? | - |
? | |
additional information | EF4-ribosome interactions during reverse translocation, overview | Escherichia coli MRE 600 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EF-G | - |
Escherichia coli |
EF4 | - |
Escherichia coli |
elongation factor 4 | - |
Escherichia coli |
elongation factor G | - |
Escherichia coli |
LepA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | an EF-G mutant lacking domains 4 and 5 shows ribosome-stimulated GTP hydrolysis activity 2.5fold slower than that of wild-type full-length EF-G and is insensitive to the effects of thiostrepton on both GTPase activity and ribosome binding | Escherichia coli |
physiological function | EF-G and EF4 perform ribosome-dependent GTP hydrolysis and bind to conserved bases in 23S rRNA and stabilize ribosomal ratcheting | Escherichia coli |