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Literature summary for 3.6.5.2 extracted from
- Functional characterization of Rho family small GTPases in Fusarium graminearum (2013), Fungal Genet. Biol., 61, 90-99.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of enzyme mutants FgRac1-G17V/D123A and FgCdc42-G15V/D121A in Saccharomyces cerevisiae strain AH109 in the yeast two-hybrid assay | Fusarium graminearum |
| recombinant expression of the enzyme | Fusarium graminearum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C192S | FgCDC42 cDNA is amplified from cDNA of PH-1 with a site mutation C192S to ensure that FgCdc42 protein cannot be prenylated and is soluble | Fusarium graminearum |
| C196S | FgRAC1 cDNA is amplified from cDNA of PH-1 with a site mutation C196S to ensure that FgRac1 protein cannot be prenylated and is soluble | Fusarium graminearum |
| G15V/D121A | constitutively active (G15V) and dominant negative mutations (D121A) are generated in the BD-FgCdc42 background | Fusarium graminearum |
| G17V/D123A | constitutively active (G17V) and dominant negative mutations (D123A) are generated in the BD-FgRac1 background | Fusarium graminearum |
| additional information | construction of deletion mutants of the isozymes, phenotypes, overview | Fusarium graminearum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Fusarium graminearum | - |
GDP + phosphate | - |
? |  |
| GTP + H2O | Fusarium graminearum PH-1 | - |
GDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium graminearum | A0A098DKM1 | isozyme FgRHO4; Rho GTPase isozyme FgRHO4 | - |
| Fusarium graminearum | A0A098E0E9 | isozyme FgRHO2; Rho GTPase isozyme FgRHO2 | - |
| Fusarium graminearum | A0A2H3FHS0 | isozyme FgRac1; Rho GTPase isozyme FgRac1 | - |
| Fusarium graminearum | A0A2H3GRH7 | isozyme FgRHO3; Rho GTPase isozyme FgRHO3 | - |
| Fusarium graminearum | I1R9M1 | isozyme FgCdc42; Rho GTPase isozyme FgCdc42 | - |
| Fusarium graminearum | Q4I2W2 | isozyme FgRHO1; Rho GTPase isozyme FgRHO1 | - |
| Fusarium graminearum PH-1 | A0A098DKM1 | isozyme FgRHO4; Rho GTPase isozyme FgRHO4 | - |
| Fusarium graminearum PH-1 | A0A098E0E9 | isozyme FgRHO2; Rho GTPase isozyme FgRHO2 | - |
| Fusarium graminearum PH-1 | A0A2H3FHS0 | isozyme FgRac1; Rho GTPase isozyme FgRac1 | - |
| Fusarium graminearum PH-1 | A0A2H3GRH7 | isozyme FgRHO3; Rho GTPase isozyme FgRHO3 | - |
| Fusarium graminearum PH-1 | I1R9M1 | isozyme FgCdc42; Rho GTPase isozyme FgCdc42 | - |
| Fusarium graminearum PH-1 | Q4I2W2 | isozyme FgRHO1; Rho GTPase isozyme FgRHO1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| conidium | - |
Fusarium graminearum | - |
| hypha | - |
Fusarium graminearum | - |
| mycelium | - |
Fusarium graminearum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Fusarium graminearum | GDP + phosphate | - |
? | |
| GTP + H2O | - |
Fusarium graminearum PH-1 | GDP + phosphate | - |
? | |
| additional information | FgRac1 interacts with the PBD domain of FgCla4 | Fusarium graminearum | ? | - |
? | |
| additional information | FgRac1 interacts with the PBD domain of FgCla4 | Fusarium graminearum PH-1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FgCdc42 | - |
Fusarium graminearum |
| FgRac1 | - |
Fusarium graminearum |
| FgRHO1 | - |
Fusarium graminearum |
| FgRHO2 | - |
Fusarium graminearum |
| FgRHO3 | - |
Fusarium graminearum |
| FgRHO4 | - |
Fusarium graminearum |
| Rho family small GTPase | - |
Fusarium graminearum |
| Rho GTPase | - |
Fusarium graminearum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the Rho GTPase family of enzymes | Fusarium graminearum |
| malfunction | all single deletion mutants of the isozymes show impaired growth, particularly in conidial morphology, when compared to the wild-type progenitor. Conidia morphology of wild-type strain PH-1 and FgRHO deletion mutants, overview | Fusarium graminearum |
| malfunction | all single deletion mutants of the isozymes show impaired growth, particularly in conidial morphology, when compared to the wild-type progenitor. Conidia morphology of wild-type strain PH-1 and FgRHO deletion mutants, septum defect and nuclear division phenotype of DELTAFgrho4 deletion mutant, overview | Fusarium graminearum |
| malfunction | all single deletion mutants of the isozymes show impaired growth, particularly in conidial morphology, when compared to the wild-type progenitor. FgRac1 deletion mutants display a precocious, multi-site germ tube formation as well as hyperbranching of hyphae. Conidia morphology of wild-type strain PH-1 and FgRHO deletion mutants, overview | Fusarium graminearum |
| physiological function | Rho GTPases have multiple cellular and metabolic functions, including vesicular trafficking and pathogenesis, as signaling molecules in fungi. Rho GTPases are known to regulate asexual development in filamentous fungi. FgRac1 interacts with FgCla4 via its PBD domain and genetically acts upstream of FgCla4 to affect hyphal growth and conidiation. Isozyme FgRac1 is important for hyphal growth and conidiation. FgRac1 negatively controls deoxynivalenol production. FgCla4 is a downstream target of FgRac1, but is dispensable for sexual development. FgRho GTPase isozymes contribute diversely to growth, conidiogenesis, sexual reproduction, deoxynivalenol production and pathogenesis | Fusarium graminearum |
| physiological function | Rho GTPases have multiple cellular and metabolic functions, including vesicular trafficking and pathogenesis, as signaling molecules in fungi. Rho GTPases are known to regulate asexual development in filamentous fungi. Isozyme FgRHO1 is essential for fungal survival. FgRho GTPase isozymes contribute diversely to growth, conidiogenesis, sexual reproduction, deoxynivalenol production and pathogenesis | Fusarium graminearum |
| physiological function | Rho GTPases have multiple cellular and metabolic functions, including vesicular trafficking and pathogenesis, as signaling molecules in fungi. Rho GTPases are known to regulate asexual development in filamentous fungi. Isozyme FgRho3 demonstrates functions only in vegetative growth and conidiation. FgRho GTPase isozymes contribute diversely to growth, conidiogenesis, sexual reproduction, deoxynivalenol production and pathogenesis | Fusarium graminearum |
| physiological function | Rho GTPases have multiple cellular and metabolic functions, including vesicular trafficking and pathogenesis, as signaling molecules in fungi. Rho GTPases are known to regulate asexual development in filamentous fungi. Rho GTPase FgRho2 is multifunctional and is involved in sexual development and pathogenesis. FgRho2 is involved in cell wall integrity. FgRho GTPase isozymes contribute diversely to growth, conidiogenesis, sexual reproduction, deoxynivalenol production and pathogenesis | Fusarium graminearum |
| physiological function | Rho GTPases have multiple cellular and metabolic functions, including vesicular trafficking and pathogenesis, as signaling molecules in fungi. Rho GTPases are known to regulate asexual development in filamentous fungi. The Rho GTPase FgCdc42 is multifunctional and is involved in sexual development and pathogenesis. Isozyme FgCdc42 is important for hyphal growth and conidiation. FgRho GTPase isozymes contribute diversely to growth, conidiogenesis, sexual reproduction, deoxynivalenol production and pathogenesis | Fusarium graminearum |
| physiological function | Rho GTPases have multiple cellular and metabolic functions, including vesicular trafficking and pathogenesis, as signaling molecules in fungi. Rho GTPases are known to regulate asexual development in filamentous fungi. The Rho GTPase FgRho4 is multifunctional and is involved in sexual development and pathogenesis. While FgRho4 is involved in cell wall integrity, and FgRho4 shows a role in nuclear division and septum formation. FgRho4 is also important for hyphal growth and conidiation. Isozyme FgRho4 plays a positive role in deoxynivalenol production. FgRho GTPase isozymes contribute diversely to growth, conidiogenesis, sexual reproduction, deoxynivalenol production and pathogenesis | Fusarium graminearum |
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