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Literature summary for 3.6.5.2 extracted from

  • Kopra, K.; Ligabue, A.; Wang, Q.; Syrjaenpaeae, M.; Blazevits, O.; Veltel, S.; van Adrichem, A.J.; Haenninen, P.; Abankwa, D.; Haermae, H.
    A homogeneous quenching resonance energy transfer assay for the kinetic analysis of the GTPase nucleotide exchange reaction (2014), Anal. Bioanal. Chem., 406, 4147-4156.
    View publication on PubMed
Search for more literature for 3.6.5.2

Activating Compound

Activating Compound Comment Organism Structure
Ect2cat RhoA activation Homo sapiens
additional information wild-type RhoA activation by Ect2cat is known to be lower than that of Ras by SOScat because of the lower affinity of GTP for wild-type RhoA than for Ras Homo sapiens
SOS980-989 peptide the recombinant SOS980-989 peptide contains the H-Ras-SOScat interaction contact region, which is known to be able to compete with SOScat for binding to H-Ras Homo sapiens
SOScat activation of Ras Homo sapiens

Application

Application Comment Organism
analysis nucleotide exchange kinetics with small GTPases can be used to obtain important information about the binding kinetics of GTP molecules under different activation states and with different activator molecules Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of GST-tagged RhoA wild-type (amino acids 1-193) in Escherichia coli Homo sapiens
recombinant expression of H-Ras wild-type (amino acids 2-189) and H-Ras mutant Q61G (amino acids 2-189) in Escherichia coli Homo sapiens
recombinant expression of K-Ras wild-type (amino acids 2-189) in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibits nucleotide binding to Ras; inhibits nucleotide binding to Ras; inhibits nucleotide binding to Ras Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis of the GTPase nucleotide exchange reaction via homogeneous quenching resonance energy transfer assay, method development, overview. Nucleotide exchange kinetics with small GTPases can be used to obtain important information about the binding kinetics of GTP molecules under different activation states and with different activator molecules Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ nucleotide binding to Ras is greatly enhanced by Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P01112 H-Ras
-
Homo sapiens P01116 K-Ras
-
Homo sapiens P61586 RhoA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?
additional information kinetic analysis of the GTPase nucleotide exchange reaction via homogeneous quenching resonance energy transfer assay, method development overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
H-Ras
-
 Homo sapiens
K-Ras
-
 Homo sapiens
RhoA
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Homo sapiens

General Information

General Information Comment Organism
malfunction misregulation of the enzyme is involved in several diseases, especially cancer Homo sapiens
physiological function small GTPases are central signaling proteins in all eukaryotic cells acting as a molecular switches that are active in the GTP-state and inactive in the GDP-state. GTP-loading is highly regulated by guanine nucleotide exchange factors Homo sapiens