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Literature summary for 3.6.5.2 extracted from

  • Zoppino, F.C.; Militello, R.D.; Slavin, I.; Alvarez, C.; Colombo, M.I.
    Autophagosome formation depends on the small GTPase Rab1 and functional ER exit sites (2010), Traffic, 11, 1246-1261.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of GFP-LC3 and either Sar1 wild-type or the mutants Sar1 H79G or Sar1 T39N in CHO cells, effect of overexpression of Sar1 and its mutants in the secretory pathway, overview. Overexpression of GFP-tagged Rab1b wild-type or mutant Rab1b in CHO cells. Overexpression of GFP-tagged Rab1b N121I mutant in Vero monkey cells and human HaCaT cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H79G a GTPase-defective Sar1 mutant. Overexpression of the GTPase-defective mutant Sar1 H79G, in its GTP-bound form, allows the development of ERES, but the transport to the Golgi apparatus is blocked because this mutant inhibits COPII coat dissociation, and its expression induces Golgi fragmentation as indicated by Rab6 dispersion and inhibits autophagosome formation Homo sapiens
N121I a dominant-negative GFP-tagged Rab1b mutant, inhibits autophagosome formation Homo sapiens
Q67L a GTPase-deficient Rab1b mutant, the distribution of Rab1b in the acidic compartments responds to inhibition of autophagy Homo sapiens
T39N a dominant-negative Sar1 mutant, expression induces Golgi fragmentation as indicated by Rab6 dispersion and inhibits autophagosome formation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-
Golgi apparatus
-
Homo sapiens 5794
-
Golgi vesicle Rab1b-labeled vesicles, distribute peripherally from the Golgi perinuclear area Homo sapiens
-
-
additional information GFP-Rab1 localizes in autophagosomes marked with either RFP-light chain 3 or endogenous light chain 3 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
two Sar1 isoforms Sar1a and Sar1b
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Synonyms

Synonyms Comment Organism
monomeric GTPase
-
Homo sapiens
Rab1B
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Homo sapiens
Sar1
-
Homo sapiens
small GTPase Rab1
-
Homo sapiens

General Information

General Information Comment Organism
malfunction overexpression of dominant-negative Sar1 and Rab1b mutants and the use of siRNAs impair autophagosome generation as determined by LC3 puncta formation and light chain 3 (LC3)-II processing. Recombinant expression of Sar1 mutants H79G or Sar1 T39N induce Golgi fragmentation Homo sapiens
additional information disruption of the secretory pathway by brefeldin A does not affect autophagosome formation Homo sapiens
physiological function Sar1 and Rab1b are monomeric GTPases that control traffic from the endoplasmic reticulum to the Golgi, Sar1 is responsible for export from the endoplasmic reticulum and is required for the initial steps in the secretory pathway, activity of both proteins is required for autophagosome formation under starvation or rapamycin treatment Homo sapiens