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Literature summary for 3.6.5.2 extracted from

  • Zhao, J.; Wang, W.N.; Tan, Y.C.; Zheng, Y.; Wang, Z.X.
    Effect of Mg2+ on the kinetics of guanine nucleotide binding and hydrolysis by Cdc42 (2002), Biochem. Biophys. Res. Commun., 297, 653-658.
    View publication on PubMed
Search for more literature for 3.6.5.2

Cloned(Commentary)

Cloned (Comment) Organism
Cdc42, expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information detailed kinetic study of the interaction of GTP with Cdc42 in the absence and presence of Mg2+, biphasic reaction Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the Mg2+ cofactor acts as a stabilizer for the bound nucleotides by slowing down the off and on rates, Mg2+ has only a minor effect on the intrinsic hydrolysis rate of GTP, kinetic study of the interaction of GTP with Cdc42 in the absence and presence of Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
GTP + H2O = GDP + phosphate mechanism, biphasic reaction Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?
GTP + H2O intrinsic GTPase reaction mechanism, biphasic reaction Homo sapiens GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Cdc42 Rho family small GTPase Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens