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Literature summary for 3.6.5.2 extracted from

  • Illenberger, D.; Schwald, F.; Pimmer, D.; Binder, W.; Maier, G.; Dietrich, A.; Gierschik, P.
    Stimulation of phospholipase C-beta2 by the Rho GTPases Cdc42Hs and Rac1 (1998), EMBO J., 17, 6241-6249.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus
expression in Sf9 cells Bos taurus

Protein Variants

Protein Variants Comment Organism
F37A Cdc42Hs, fails to stimulate phospholipase C beta2 protein Bos taurus
Y40C Cdc42Hs, fails to stimulate phospholipase C beta2 protein Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 Cdc42Hs, SDS-PAGE Bos taurus
23000
-
 complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Homo sapiens
26000
-
 complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Homo sapiens
26000
-
 complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ir
GTP + H2O Bos taurus
-
 GDP + phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes, affinity chromatography Homo sapiens
recombinant enzymes, affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
neutrophil
-
 Homo sapiens
-
neutrophil Cdc42Hs, Rac1, members of Rho GTPase subfamily Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ir
GTP + H2O
-
 Bos taurus GDP + phosphate
-
 ir
guanosine 5'-O-(3-thiotriphosphate) + H2O poorly hydrolyzable, GTP[S]-activated Rac1 and Cdc42, but not RhoA, stimulate activity of phospholipase C-beta2 protein in complex with Rho GDP dissociation inhibitor protein LyGDL Homo sapiens guanosine 5'-O-diphosphate + thiophosphate
-
 ?
guanosine 5'-O-(3-thiotriphosphate) + H2O poorly hydrolyzable, GTP[S]-activated Rac1 and Cdc42, but not RhoA, stimulate activity of phospholipase C-beta2 protein in complex with Rho GDP dissociation inhibitor protein LyGDL Bos taurus guanosine 5'-O-diphosphate + thiophosphate
-
 ?

Subunits

Subunits Comment Organism
dimer complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Homo sapiens
dimer complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Bos taurus

Synonyms

Synonyms Comment Organism
Cdc42Hs Rho subfamily Homo sapiens
Rac1 Rho subfamily Homo sapiens
Rac1 Rho subfamily Bos taurus
RhoA Rho subfamily Homo sapiens
RhoA Rho subfamily Bos taurus