Literature summary for 3.6.5.1 extracted from

De Vendittis, E.; Adinolfi, B.S.; Amatruda, M.R.; Raimo, G.; Masullo, M.; Bocchini, V.
The A26G replacement in the consensus sequence A-X-X-X-X-G-K-[T,S] of the guanine nucleotide binding site activates the intrinsic GTPase of the elongation factor 2 from the archaeon Sulfolobus solfataricus (1999), Eur. J. Biochem., 262, 600-605.

Search for more literature for 3.6.5.1

Activating Compound

Activating Compound	Comment	Organism	Structure
ethylene glycol	-	Saccharolobus solfataricus
additional information	GTPase activity is stimulated by ribosomes	Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment)	Organism
into the pT7-7 vector for expession in Escherichia coli JM109DE3 cells	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
A26G	mutant, rate of poly(U)-directed poly(Phe) synthesis and the ribosome-dependent GTPase activity are decreased, the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol is enhanced	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
GDP	competitive inhibition	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0008	-	GTP	A26GSsEF-2, 40% glycol	Saccharolobus solfataricus
0.001	-	GTP	SsEF-2, 40% glycol	Saccharolobus solfataricus
0.0055	-	GTP	A26GSsEF-2, 10% glycol	Saccharolobus solfataricus
0.0096	-	GTP	SsEF-2, 10% glycol	Saccharolobus solfataricus
0.0194	-	GTP	A26GSsEF-2, 0% glycol	Saccharolobus solfataricus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
ribosome	-	Saccharolobus solfataricus	5840	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Saccharolobus solfataricus	-	GDP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Saccharolobus solfataricus	GDP + phosphate	-	ir

Synonyms

Synonyms	Comment	Organism
EF-2	-	Saccharolobus solfataricus
EF-G	-	Saccharolobus solfataricus
elongation factor 2	-	Saccharolobus solfataricus
heterotrimeric G-protein GTPase	-	Saccharolobus solfataricus
SsEF-2	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	activity assay	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.2	-	GTP	SsEF-2, 10% glycol	Saccharolobus solfataricus
1.9	-	GTP	SsEF-2, 40% glycol	Saccharolobus solfataricus
7.2	-	GTP	A26GSsEF-2, 10% glycol	Saccharolobus solfataricus
8	-	GTP	A26GSsEF-2, 0% glycol	Saccharolobus solfataricus
9.9	-	GTP	A26GSsEF-2, 40% glycol	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	activity assay	Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0003	-	GDP	SsEF-2, 40% glycol	Saccharolobus solfataricus
0.0005	-	GDP	A26GSsEF-2, 40% glycol	Saccharolobus solfataricus
0.0074	-	GDP	A26GSsEF-2, 0% glycol	Saccharolobus solfataricus

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Release 2023.1 (January 2023)