Literature summary for 3.6.5.1 extracted from

Thomas, C.J.; Du, X.; Li, P.; Wang, Y.; Ross, E.M.; Sprang, S.R.
Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunit (2004), Proc. Natl. Acad. Sci. USA, 101, 7560-7565.

Search for more literature for 3.6.5.1

Activating Compound

Activating Compound	Comment	Organism	Structure
RGS4	-	Mammalia

Cloned(Commentary)

Cloned (Comment)	Organism
into vector for expression in Escherichia coli	Mammalia

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of mutant K180P-Galphai1 bound to a GTP analog	Mammalia

Protein Variants

Protein Variants	Comment	Organism
G202A	mutation of Galphai1 accelerates the rates of GTP hydrolysis and conformational change	Mammalia
G202A, K180P	mutations of Galphai1 accelerate the rates of GTP hydrolysis	Mammalia
K180A	mutant of Galphai1, rate of GTP hydrolysis similar to wild-type	Mammalia
K180P	mutation of Galphai1 increases the rate of conformational change and decreases the rates of GTP hydrolysis	Mammalia

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Mammalia

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Mammalia	-	GDP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mammalia	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a Ni-NTA column	Mammalia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Mammalia	GDP + phosphate	-	?

Subunits

Subunits	Comment	Organism
heterotrimer	Galpha subunit, GDP-bound inactive, GTP-bound active and betagamma dimer	Mammalia

Synonyms

Synonyms	Comment	Organism
GTP phosphohydrolase	-	Mammalia
heterotrimeric G-protein GTPase	-	Mammalia

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	single-turnover GTPase assay	Mammalia

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Release 2023.1 (January 2023)