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Literature summary for 3.6.4.B8 extracted from

  • Chen, Y.H.; Lin, Y.; Yoshinaga, A.; Chhotani, B.; Lorenzini, J.L.; Crofts, A.A.; Mei, S.; Mackie, R.I.; Ishino, Y.; Cann, I.K.
    Molecular analyses of a three-subunit euryarchaeal clamp loader complex from Methanosarcina acetivorans (2009), J. Bacteriol., 191, 6539-6549.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression of RFCS1Ma and RFCS2Ma as N-terminal His6-tagged proteins Methanosarcina acetivorans

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans Q8TSX5 and Q8TUC8 and Q8TPU4 Q8TSX5: Replication factor C small subunit, Q8TUC8: Replication factor C subunit, Q8TPU4: Replication factor C large subunit
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Methanosarcina acetivorans DSM 2834 Q8TSX5 and Q8TUC8 and Q8TPU4 Q8TSX5: Replication factor C small subunit, Q8TUC8: Replication factor C subunit, Q8TPU4: Replication factor C large subunit
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Subunits

Subunits Comment Organism
oligomer Methanosarcina acetivorans clamp loader comprises two different small subunits (RFCS1 and RFCS2) and a large subunit (RFCL). RFCS1, RFCS2, and RFCL form a stable complex with a stoichiometric ratio of 3:1:1 Methanosarcina acetivorans