Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ssDNA | ATPase activity of RadA is drastically stimulated by ssDNA and to a lesser extent by dsDNA. The activity increases linearly with ssDNA concentration until a saturation plateau, at which the apparent binding stoichiometry is 3 nucleotides for 1 RadA | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38400 | - |
x * 38400, SDS-PAGE, calculated from sequence | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Pyrococcus furiosus | DNA-dependent ATPase, D-loop formation, and strand exchange activities. The enzyme is involved in homologous recombination | ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | O74036 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | DNA-dependent ATPase, D-loop formation, and strand exchange activities. The enzyme is involved in homologous recombination | Pyrococcus furiosus | ADP + phosphate | - |
? | |
ATP + H2O | DNA-dependent ATPase, D-loop formation, and strand exchange activities | Pyrococcus furiosus | ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38400, SDS-PAGE, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
RadA | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
ATP | pH 7.5, 70°C | Pyrococcus furiosus | |
0.017 | - |
ATP | pH 7.5, 70°C, addition of dsDNA | Pyrococcus furiosus | |
0.0345 | - |
ATP | pH 7.5, 70°C, addition of ssDNA | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pyrococcus furiosus |
Organism | Comment | Expression |
---|---|---|
Pyrococcus furiosus | expression of radA might be constitutive | additional information |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in homologous recombination | Pyrococcus furiosus |