Literature summary for 3.6.4.B1 extracted from

Tanaka, K.; Umeki, N.; Toshiaki, M.; Fujimoto, Z.; Maruta, S.
Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K16 (2010), Biochem. Biophys. Res. Commun., 401, 251-256.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLys strain	Oryza sativa

Crystallization (Commentary)

Crystallization (Comment)	Organism
K16MD, the rice kinesin motor domain complexed with Mg-ADP is crystallized by the sitting drop vapor diffusion method at 19.85°C, resulting in a 2.4 A resolution. The ADP-free form of the novel rice-plant-specific kinesin K16 is very stable, whereas the ADP-free form of conventional kinesins is labile. The overall structure of K16MD is similar to that of conventional kinesin motors domains, as expected from the high amino acid sequence similarity of 43.2%. The position and length of the L5, L11m and L12 loops, which are key functional regions, are different from those observed in conventional kinesins. Moreover, the neck-lonker region of the ADP-bound K16MD shows an ordered confirmation at a position quite different from that of conventional kinesins	Oryza sativa

Crystallization (Comment)

Organism

K16MD, the rice kinesin motor domain complexed with Mg-ADP is crystallized by the sitting drop vapor diffusion method at 19.85°C, resulting in a 2.4 A resolution. The ADP-free form of the novel rice-plant-specific kinesin K16 is very stable, whereas the ADP-free form of conventional kinesins is labile. The overall structure of K16MD is similar to that of conventional kinesin motors domains, as expected from the high amino acid sequence similarity of 43.2%. The position and length of the L5, L11m and L12 loops, which are key functional regions, are different from those observed in conventional kinesins. Moreover, the neck-lonker region of the ADP-bound K16MD shows an ordered confirmation at a position quite different from that of conventional kinesins

Oryza sativa

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	-	-	-

Synonyms

Synonyms	Comment	Organism
K16MD	-	Oryza sativa
kinesin K-16	novel rice-plant-specific kinesin K16	Oryza sativa
kinesin K16MD	-	Oryza sativa

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Release 2023.1 (January 2023)