Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ist1 | addition of the endosomal sorting complex required for the transport III (ESCRT-III) subunit binding partner of Ist1, Did2, converts ESCRT-III subunit Ist1 from an inhibitor to a stimulator of the enzyme (maximal stimulation at 0.008 or less mM). Mutants Ist1(L168A/Y172A) and Ist1(K135A) hyperstimulate enzyme ATPase activity | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ist1 | subunit of endosomal sorting complex required for the transport III, maximal inhibition at 0.008 or less mM | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Saccharomyces cerevisiae | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 75 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Vps4 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme remodels and disassembles endosomal sorting complex required for transport III | Saccharomyces cerevisiae |