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Literature summary for 3.6.4.6 extracted from

  • Tan, J.; Davies, B.A.; Payne, J.A.; Benson, L.M.; Katzmann, D.J.
    Conformational changes in the endosomal sorting complex required for the Transport III subunit ist1 lead to distinct modes of ATPpase Vps4 regulation (2015), J. Biol. Chem., 290, 30053-30065 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Ist1 addition of the endosomal sorting complex required for the transport III (ESCRT-III) subunit binding partner of Ist1, Did2, converts ESCRT-III subunit Ist1 from an inhibitor to a stimulator of the enzyme (maximal stimulation at 0.008 or less mM). Mutants Ist1(L168A/Y172A) and Ist1(K135A) hyperstimulate enzyme ATPase activity Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Ist1 subunit of endosomal sorting complex required for the transport III, maximal inhibition at 0.008 or less mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Saccharomyces cerevisiae
-
ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Saccharomyces cerevisiae ADP + phosphate
-
?

Synonyms

Synonyms Comment Organism
Vps4
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function the enzyme remodels and disassembles endosomal sorting complex required for transport III Saccharomyces cerevisiae