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Literature summary for 3.6.4.6 extracted from

  • Barajas, D.; Martin, I.F.; Pogany, J.; Risco, C.; Nagy, P.D.
    Noncanonical role for the host Vps4 AAA+ ATPase ESCRT protein in the formation of Tomato bushy stunt virus replicase (2014), PLoS Pathog., 10, e1004087.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.6

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of GST- and His6-tagged Vps4p and Vps4101–437 in Saccharomyces cerevisiae strain BY4741 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of a Vps4p deletion mutant Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Saccharomyces cerevisiae 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins ?
-
 ?
additional information Saccharomyces cerevisiae BY4741 the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4741
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST- and His6-tagged Vps4p and Vps4101–437 from Saccharomyces cerevisiae strain BY4741 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins Saccharomyces cerevisiae ?
-
 ?
additional information the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins Saccharomyces cerevisiae BY4741 ?
-
 ?

Synonyms

Synonyms Comment Organism
Vps4p
-
 Saccharomyces cerevisiae
Vps4p AAA ATPase
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction deletion of Vps4p in yeast leads to the formation of crescent-like membrane structures instead of the characteristic spherule and vesicle-like structures Saccharomyces cerevisiae
physiological function Vps4p is required for the formation of spherule-like structures induced by tombusvirus replication proteins in yeast. Key role for the conserved Vps4p AAA ATPase, whose canonical function is to disassemble the ESCRT complexes, i.e. endosomal sorting complexes required for transport, and recycle them from the membranes back to the cytosol. Vps4p is recruited to become a permanent component of the viral replicase complexes, it is co-localized with the viral dsRNA and contacts the viral (+)RNA in the intracellular membrane Saccharomyces cerevisiae