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Literature summary for 3.6.4.6 extracted from

  • Vivona, S.; Cipriano, D.J.; OLeary, S.; Li, Y.H.; Fenn, T.D.; Brunger, A.T.
    Disassembly of all SNARE complexes by N-ethylmaleimide-sensitive factor (NSF) is initiated by a conserved 1:1 interaction between alpha-soluble NSF attachment protein (SNAP) and SNARE complex (2013), J. Biol. Chem., 288, 24984-24991.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.6

Activating Compound

Activating Compound Comment Organism Structure
SNARE complex SNARE-stimulated ATP hydrolysis, all four SNARE complexes similarly stimulate the steady-state rate of NSF-catalyzed ATP hydrolysis activity, suggesting that the stimulation of ATPase activity is independent of SNARE primary sequence variation and N-terminal domain architecture Cricetulus griseus

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged enzyme NSF in Escherichia coli strain BL21(DE3), coexpression of alpha-SNAP, VAMP2(1-96), syntaxin1A(1-265)-C145S/S249C/K253C, syntaxin1A(191-265)-C145S/S249C/K253C, and SNAP25A Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Cricetulus griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Cricetulus griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Cricetulus griseus
-
 ADP + phosphate
-
 ?
additional information Cricetulus griseus modeling of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding. Subsequent additional alpha-SNAP binding events may occur as part of a processive disassembly mechanism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme NSF from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Cricetulus griseus ADP + phosphate
-
 ?
additional information modeling of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding. Subsequent additional alpha-SNAP binding events may occur as part of a processive disassembly mechanism Cricetulus griseus ?
-
 ?

Synonyms

Synonyms Comment Organism
N-ethylmaleimide-sensitive factor
-
 Cricetulus griseus
NSF
-
 Cricetulus griseus
NSF ATPase
-
 Cricetulus griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Cricetulus griseus

General Information

General Information Comment Organism
metabolism vesicle trafficking in eukaryotic cells is facilitated by SNARE-mediated membrane fusion. The ATPase N-ethylmaleimide-sensitive factor, NSF, and the adaptor protein soluble NSF attachment protein, alpha-SNAP, disassemble all SNARE complexes formed throughout different pathways Cricetulus griseus
physiological function vesicle trafficking in eukaryotic cells is facilitated by SNARE-mediated membrane fusion. The ATPase N-ethylmaleimide-sensitive factor, NSF, and the adaptor protein soluble NSF attachment protein, alpha-SNAP, disassemble all SNARE complexes formed throughout different pathways. SNARE-stimulated ATP hydrolysis. Modeling of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding. Subsequent additional alpha-SNAP binding events may occur as part of a processive disassembly mechanism Cricetulus griseus