Literature summary for 3.6.4.6 extracted from

Hartmann, C.; Chami, M.; Zachariae, U.; de Groot, B.L.; Engel, A.; Gruetter, M.G.
Vacuolar protein sorting: two different functional states of the AAA-ATPase Vps4p (2008), J. Mol. Biol., 377, 352-363.

Search for more literature for 3.6.4.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, at 19°C against a reservoir containing 0.1 M MES buffer (pH 5.9-6.5) and 0.8-1.9 M MgSO4	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
Q216A	monomeric mutant form	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P52917	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-chelating column chromatography and Superdex gel filtration	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Saccharomyces cerevisiae	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	Vps4p dimers form two distinct heptameric rings and accommodate AAA cassettes in a head-to-head fashion, X-ray crystallography	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
AAA-ATPase	-	Saccharomyces cerevisiae
vacuolar protein sorting 4 protein	-	Saccharomyces cerevisiae
Vps4p	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)