Literature summary for 3.6.4.6 extracted from

Briggs, L.C.; Baldwin, G.S.; Miyata, N.; Kondo, H.; Zhang, X.; Freemont, P.S.
Analysis of nucleotide binding to p97 reveals the properties of a tandem AAA hexameric ATPase (2008), J. Biol. Chem., 283, 13745-13752.

Search for more literature for 3.6.4.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
K251A	the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme	Rattus norvegicus
K251A	the Walker A mutation to the D1 domain alone shows a moderate decrease in ATPase activity, has reduced affinity for nucleotide in the D1 domain and so contains little prebound ADP	Rattus norvegicus
K524A	the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme	Rattus norvegicus
R359A	the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme	Rattus norvegicus
R635A	the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P46462	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Ni2+-chelating column chromatography	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Rattus norvegicus	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
homohexamer	x-ray crystallography	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
peptide p97	essential chaperone in endoplasmic reticulum-associated degradation and organelle biogenesis, contains two ATPases associated with various cellular activities domains (D1 and D2)	Rattus norvegicus
vasolin-containing protein	-	Rattus norvegicus
VCP	-	Rattus norvegicus

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Release 2023.1 (January 2023)