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Literature summary for 3.6.4.6 extracted from
- The beta domain is required for Vps4p oligomerization into a functionally active ATPase (2006), FEBS J., 273, 2357-2373.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the vectors YCplac111, pLexA, pET11d and pB42AD are used for cloning and expression of wild-type protein, single domains and various mutants | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endosome | - |
Saccharomyces cerevisiae | 5768 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Saccharomyces cerevisiae | - |
ADP + phosphate | - |
? |  |
| ATP + H2O | Saccharomyces cerevisiae | - |
ADP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
ir | |
| ATP + H2O | Vsp4 is an AAA ATPase which plays a key role in sorting of multivesicular bodies and facilitates transport through endosomes. The beta-domain of Vsp4p plays a critical role in the formation of a Vsp4p ATPase complex that is functional in vivo. The formation of the latter may be a prerequisite for interaction with Vta1p | Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Csc1p | - |
Saccharomyces cerevisiae |
| Did6p | - |
Saccharomyces cerevisiae |
| End13p | - |
Saccharomyces cerevisiae |
| Grd13p | - |
Saccharomyces cerevisiae |
| Vpl4p | - |
Saccharomyces cerevisiae |
| Vps4p | - |
Saccharomyces cerevisiae |
| Vpt10p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
ATPase activity assay | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
ATPase activity assay | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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