Cloned (Comment) | Organism |
---|---|
gene ISE2, quantitative RT-PCR expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | virus-induced ISE2 gene silencing, TRV constructs are introduced into Agrobacterium strain GV3101 for plant infiltrations. Loss of ISE2 compromises C-to-U RNA editing at specific sites. Total RNA is isolated from leaves of the same age as the ISE2-SUP leaves analyzed, and RNA editing at the six candidate ISE2-specific sites and several unaffected sites is measured. The clpP1-559 site exhibited reduced RNA editing efficiency in both the ISE2-SUP and the var2-2 leaves, but the extent of RNA editing in ISE2-SUP leaves (25%) is less than half of that in var2-2 leaves (59%). Thus, depletion of ISE2 has a more drastic effect on the RNA editing efficiency at the clpP1-559 site than depletion of FTSH2. Similarly, while the var2-2 mutant displays decreased RNA editing at petL-5 and rpoA-200, the decrease at those sites is more drastic in ISE2-SUP leaves than in var2-2 leaves. Accumulation of chloroplast mRNAs is altered in the absence of ISE2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Arabidopsis thaliana | - |
ADP + phosphate | - |
? | |
ATP + H2O | Arabidopsis thaliana Col-0 | - |
ADP + phosphate | - |
? | |
additional information | Arabidopsis thaliana | ISE2 associates with numerous chloroplast RNA species, chloroplast rRNAs are potential ISE2 substrates. ISE2 associates with transcripts containing C-to-U editing sites | ? | - |
- |
|
additional information | Arabidopsis thaliana Col-0 | ISE2 associates with numerous chloroplast RNA species, chloroplast rRNAs are potential ISE2 substrates. ISE2 associates with transcripts containing C-to-U editing sites | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | B9DFG3 | - |
- |
Arabidopsis thaliana Col-0 | B9DFG3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Arabidopsis thaliana | ADP + phosphate | - |
? | |
ATP + H2O | - |
Arabidopsis thaliana Col-0 | ADP + phosphate | - |
? | |
additional information | ISE2 associates with numerous chloroplast RNA species, chloroplast rRNAs are potential ISE2 substrates. ISE2 associates with transcripts containing C-to-U editing sites | Arabidopsis thaliana | ? | - |
- |
|
additional information | ISE2 associates with numerous chloroplast RNA species, chloroplast rRNAs are potential ISE2 substrates. ISE2 associates with transcripts containing C-to-U editing sites | Arabidopsis thaliana Col-0 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
increased size exclusion limit2 | - |
Arabidopsis thaliana |
ISE2 | - |
Arabidopsis thaliana |
RNA helicase ISE2 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | ISE2 is a non-canonical Ski2-like RNA helicase that represents a separate sub-clade unique to green photosynthetic organisms. ISE2's evolutionary conservation may be explained by its numerous roles in regulating chloroplast gene expression | Arabidopsis thaliana |
malfunction | chloroplasts in leaves with reduced ISE2 expression show reduced thylakoid contents and increased stromal volume, indicative of defective development. Embryonic-lethal phenotype of Arabidopsis ise2 mutants. Changes observed in RNA editing in tissues with decreased ISE2 levels (ISE2-SUP leaves) are due to the specific effects of ISE2 and not due to general chlorosis, RNA editing is also measured in chlorotic leaves resulting from disruption of other genes | Arabidopsis thaliana |
physiological function | increased size exclusion limit2 (ISE2) is a chloroplast-localized RNA helicase that is indispensable for proper plant development. Enzyme ISE2 is required for multiple chloroplast RNA processing steps in Arabidopsis thaliana. ISE2 is required for the splicing of group II introns from chloroplast transcripts. ISE2 is required for site-specific chloroplast C-to-U RNA editing. ISE2 is required for chloroplast rRNA processing and splicing of group II introns. And ISE2 is required for accumulation of 30S and 50S ribosomal RNAs and chloroplast-encoded proteins | Arabidopsis thaliana |