Cloned (Comment) | Organism |
---|---|
gene PA2840, DNA and amino acid sequence dtermiantion and analysis, recombinant expression of wild-type and mutant enzymes in Eescherichia coli strain Tuner (DE3), quantitative reverse transcriptase PCR | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E168A | site-directed mutagenesis, the mutation eliminates ATP hydrolysis and helicase activity, and the mutant is unable to restore PexsD-lacZ activity to levels observed with wild-type DeaD | Pseudomonas aeruginosa |
E168Q | site-directed mutagenesis, the mutation eliminates ATP hydrolysis and helicase activity, and the mutant is unable to restore PexsD-lacZ activity to levels observed with wild-type DeaD | Pseudomonas aeruginosa |
additional information | construction of an deletion mutant DELTAdeaD | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Pseudomonas aeruginosa | - |
ADP + phosphate | - |
? | |
ATP + H2O | Pseudomonas aeruginosa ATCC 15692 | - |
ADP + phosphate | - |
? | |
ATP + H2O | Pseudomonas aeruginosa 1C | - |
ADP + phosphate | - |
? | |
ATP + H2O | Pseudomonas aeruginosa PRS 101 | - |
ADP + phosphate | - |
? | |
ATP + H2O | Pseudomonas aeruginosa DSM 22644 | - |
ADP + phosphate | - |
? | |
ATP + H2O | Pseudomonas aeruginosa CIP 104116 | - |
ADP + phosphate | - |
? | |
ATP + H2O | Pseudomonas aeruginosa LMG 12228 | - |
ADP + phosphate | - |
? | |
ATP + H2O | Pseudomonas aeruginosa JCM 14847 | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I003 | - |
- |
Pseudomonas aeruginosa 1C | Q9I003 | - |
- |
Pseudomonas aeruginosa ATCC 15692 | Q9I003 | - |
- |
Pseudomonas aeruginosa CIP 104116 | Q9I003 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9I003 | - |
- |
Pseudomonas aeruginosa JCM 14847 | Q9I003 | - |
- |
Pseudomonas aeruginosa LMG 12228 | Q9I003 | - |
- |
Pseudomonas aeruginosa PRS 101 | Q9I003 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Pseudomonas aeruginosa | ADP + phosphate | - |
? | |
ATP + H2O | - |
Pseudomonas aeruginosa ATCC 15692 | ADP + phosphate | - |
? | |
ATP + H2O | - |
Pseudomonas aeruginosa 1C | ADP + phosphate | - |
? | |
ATP + H2O | - |
Pseudomonas aeruginosa PRS 101 | ADP + phosphate | - |
? | |
ATP + H2O | - |
Pseudomonas aeruginosa DSM 22644 | ADP + phosphate | - |
? | |
ATP + H2O | - |
Pseudomonas aeruginosa CIP 104116 | ADP + phosphate | - |
? | |
ATP + H2O | - |
Pseudomonas aeruginosa LMG 12228 | ADP + phosphate | - |
? | |
ATP + H2O | - |
Pseudomonas aeruginosa JCM 14847 | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent RNA helicase | - |
Pseudomonas aeruginosa |
DeaD | - |
Pseudomonas aeruginosa |
RNA helicase DeaD | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the DEAD box family of RNA helicases that have roles in mRNA degradation and ribosome biogenesis, an additional role in gene regulation is determined for bacteria. DEAD box helicases are thought to promote translation by enhancing ribosomal recruitment | Pseudomonas aeruginosa |
malfunction | transposon disruptions within gene PA2840, which encodes a homolog of the Escherichia coli RNA-helicase DeaD, significantly reduces Pseudomonas aeruginosa type III secretion system (T3SS) gene expression, the activity of an exsA translational fusion is reduced in a deaD mutant. In addition, exsA expression in trans fails to restore T3SS gene expression in a deaD mutant | Pseudomonas aeruginosa |
metabolism | DeaD RNA-helicase regulates ExsA synthesis and the T3SS expression. DeaD-based regulation of T3SS gene expression does not involve the Gac/Rsm system | Pseudomonas aeruginosa |
additional information | the DEAD motif is critical for T3SS gene expression. DeaD-dependent activation of ExsA translation is specific to the exsA coding sequence and native Shine-Dalgarno sequence | Pseudomonas aeruginosa |
physiological function | enzyme DeaD stimulates ExsA synthesis at the posttranscriptional level, DeaD promotes T3SS expression. ExsA is the master regulator of T3SS transcription. The Pseudomonas aeruginosa type III secretion system (T3SS) is a primary virulence factor important for phagocytic avoidance, disruption of host cell signaling, and host cell cytotoxicity. The expression, synthesis, and activity of ExsA is tightly regulated by both intrinsic and extrinsic factors. Intrinsic regulation consists of the ExsECDA partner-switching cascade, while extrinsic factors include global regulators that alter exsA transcription and/or translation. DeaD relaxes mRNA secondary structure to promote exsA translation and altering the mRNA sequence of exsA or the native exsA Shine-Dalgarno sequence relieves the requirement for DeaD in vivo. Regulatory mechanism for DeaD, overview. DeaD promotes ExsA synthesis at a posttranscriptional level by activating ExsA translation. The RNA helicase plays a critical role in promoting ExsA synthesis | Pseudomonas aeruginosa |