Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, strains XL-1 Blue, Rosetta (DE3), M15 (pREP4), vector pET-21-2c, kinetics of NS3 protein accumulation upon its expression in Escherichia coli at 25°C for 1-5 h shown | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',3'-ddATP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
2',3'-ddGTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
2',3'-ddTTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
2'-dATP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
2'-deoxythymidine 5'-phosphoryl-beta,gamma-hypophosphate | i.e. ppopT, dTTP analogue, most efficient inhibitor of NTPase activity among nucleotide derivaties, inhibits the ATP-dependent helicase reaction and also the ATP-independent duplex unwinding, structure of nucleic base and ribose fragment of NTP molecule have a slight effects on inhibitory properties | Hepacivirus C | |
2'-dGTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
2'-dTTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
3'-dATP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
3'-dGTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
3'-dUTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
ADP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
AMP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
beta,gamma-methylene-ATP | efficient inhibitor, like the N1-oxides N1-O-ATP and N1-OH-ITP | Hepacivirus C | |
GTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
Imidodiphosphate | maximal inhibitory activity among diphosphate analogues, non-catalytic and catalytic conditions, inhibits the ATP-dependent helicase reaction but no effect on the ATP-independent duplex unwinding, structure of nucleic base and ribose fragment of NTP molecule have a slight effects on inhibitory properties | Hepacivirus C | |
KCl | slight decrease of activity in presence of | Hepacivirus C | |
N1-O-ATP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
N1-OH-ITP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
UTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.163 | - |
ATP | addition of polyuridylate lowers Km for the ATP substrate | Hepacivirus C | |
0.256 | - |
ATP | wild-type | Hepacivirus C |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activity 3-5-fold lower when magnesium ions are replaced by | Hepacivirus C | |
Mg2+ | maximal NTPase activity achieved in the presence of 1.5-2 mM MgCl2 | Hepacivirus C | |
Mn2+ | activity 3-5-fold lower when magnesium ions are replaced by | Hepacivirus C | |
Ni2+ | activity 3-5-fold lower when magnesium ions are replaced by | Hepacivirus C | |
Zn2+ | activity 3-5-fold lower when magnesium ions are replaced by | Hepacivirus C |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Hepacivirus C | NTPase activity analyzed, ambiguous helicase activity, enzyme capable for unwinding RNA and DNA | ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, recombinant protein | Hepacivirus C |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
structure of nucleic base and ribose fragment of NTP molecule has a slight effect on inhibitory properties | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | NTPase activity analyzed, ambiguous helicase activity, enzyme capable for unwinding RNA and DNA | Hepacivirus C | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
non structural protein 3 | ambiguous | Hepacivirus C |
protein NS3 | ambiguous | Hepacivirus C |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.097 | - |
2'-deoxythymidine 5'-phosphoryl-beta,gamma-hypophosphate | i.e. ppopT, dTTP analogue, inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.109 | - |
N1-OH-ITP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.116 | - |
2'-dTTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.116 | - |
2',3'-ddATP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.141 | - |
3'-dATP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.145 | - |
beta,gamma-methylene-ATP | efficient inhibitor, like the N1-oxides N1-O-ATP and N1-OH-ITP | Hepacivirus C | |
0.205 | - |
N1-O-ATP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.26 | - |
3'-dUTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.277 | - |
2'-dGTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.291 | - |
2'-dATP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.298 | - |
2',3'-ddTTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.443 | - |
3'-dGTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.576 | - |
GTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
0.721 | - |
2',3'-ddGTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
1.3 | - |
ADP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
1.46 | - |
UTP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C | |
5 | - |
AMP | inhibition of NTPase activity of NS3 protein by NTP derivatives | Hepacivirus C |