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Literature summary for 3.6.4.13 extracted from

  • Singh, M.; Srivastava, K.K.; Bhattacharya, S.M.
    Molecular cloning and characterization of a novel immunoreactive ATPase/RNA helicase in human filarial parasite Brugia malayi (2009), Parasitol. Res., 104, 753-761.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Deoxyribonucleotides ATPase activity of the bacterially expressed BmL3-helicase is triggered by both the ssRNA and the dsRNA and, to a much lesser extent, by the ssDNA and dsDNA Brugia malayi
Double-stranded DNA weak stimulation Brugia malayi
Double-stranded RNA ATPase activity of the recombinant BmL3-helicase is strongly stimulated by dsRNA Brugia malayi
ribonucleotides ATPase activity of the bacterially expressed BmL3-helicase is triggered by both the ssRNA and the dsRNA and, to a much lesser extent, by the ssDNA and dsDNA, recombinant BmL3-helicase is strongly stimulated by dsRNA Brugia malayi
single-stranded DNA weak stimulation Brugia malayi
Single-stranded RNA stimulates Brugia malayi

Cloned(Commentary)

Cloned (Comment) Organism
expression of the N-terminally His-tagged enzyme as soluble protein in Escherichia coli strain BL21(DE3) Brugia malayi
overexpressed as His-tag fusion protein in Escherichia coli Brugia malayi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Brugia malayi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Brugia malayi
-
ADP + phosphate
-
?
additional information Brugia malayi DEAD box proteins are putative RNA unwinding proteins, BmL3-helicase also is a DEAD box RNA helicase ?
-
?
RNA + H2O Brugia malayi helicase/unwinding activity, either ATP or dATP is required for the unwinding activity ?
-
?

Organism

Organism UniProt Comment Textmining
Brugia malayi
-
-
-
Brugia malayi EF409381
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Brugia malayi

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
Brugia malayi
-
additional information presence of BmL3-helicase in various life stages of Brugia malayi Brugia malayi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Brugia malayi ADP + phosphate
-
?
ATP + H2O either ATP or dATP is required for the unwinding activity Brugia malayi ADP + phosphate
-
?
dATP + H2O either ATP or dATP is required for the unwinding activity Brugia malayi dADP + phosphate
-
?
additional information DEAD box proteins are putative RNA unwinding proteins, BmL3-helicase also is a DEAD box RNA helicase Brugia malayi ?
-
?
RNA + H2O helicase/unwinding activity, either ATP or dATP is required for the unwinding activity Brugia malayi ?
-
?

Synonyms

Synonyms Comment Organism
ATP/dATP-dependent RNA helicase
-
Brugia malayi
ATPase/RNA helicase
-
Brugia malayi
BmL3-helicase
-
Brugia malayi
DEAD box RNA helicase
-
Brugia malayi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Brugia malayi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Brugia malayi