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Literature summary for 3.6.4.13 extracted from

  • Flores-Rozas, H.; Hurwitz, J.
    Characterization of a new RNA helicase from nuclear extracts of HeLa cells which translocates in the 5' to 3' direction. (1993), J. Biol. Chem., 268, 21372-21383.
    View publication on PubMed
Search for more literature for 3.6.4.13

Activating Compound

Activating Compound Comment Organism Structure
poly(C) stimulates ATPase and dATPase activity Homo sapiens
Single-stranded RNA RNA helicase catalyzes the unwinding of duplex RNA and RNA*DNA hybrids provided that single-stranded RNA is available for the helicase to bind Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
KCl RNA-dependent ATPase activity is sensitive to high salt concentrations. Maximal activity is obtained in the absence of KCl, and it is inhibited 50% at 0.1 M KC1, completely inhibited at 0.3 M KCl Homo sapiens
NEM 5 mM, ATPase activity is blocked Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement for divalent ions for ATP hydrolysis is specific for Mg2+ and is not supported by Mn2+ and Ca2+. Half-maximum activity is achieved with concentrations of Mg2+ of 0.120 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 x * 100000, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O the enzyme displaces partial duplex RNA exclusively in a 5' to 3' direction. This reaction is supported by ATP and dATP at relatively high concentrations. The enzyme displays only ATPase and dATPase activity. RNA helicase catalyzes the unwinding of duplex RNA and RNA*DNA hybrids provided that single-stranded RNA is available for the helicase to bind Homo sapiens ADP + phosphate
-
 ?
dATP + H2O the enzyme displaces partial duplex RNA exclusively in a 5' to 3' direction. This reaction is supported by ATP and dATP at relatively high concentrations. The enzyme displays only ATPase and dATPase activity. RNA helicase catalyzes the unwinding of duplex RNA and RNA*DNA hybrids provided that single-stranded RNA is available for the helicase to bind Homo sapiens dADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, gel filtration Homo sapiens