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Literature summary for 3.6.4.13 extracted from

  • Cordin, O.; Tanner, N.K.; Doere, M.; Linder, P.; Banroques, J.
    The newly discovered Q motif of DEAD-box RNA helicases regulates RNA-binding and helicase activity (2004), EMBO J., 23, 2478-2487.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.13

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
F162A kcat/KM for ATP is 1% of wild-type value Saccharomyces cerevisiae
F162L kcat/KM for ATP is 25% of wild-type value Saccharomyces cerevisiae
Q169A kcat/KM for ATP is 0.3% of wild-type value Saccharomyces cerevisiae
Q169E kcat/KM for ATP is 0.4% of wild-type value Saccharomyces cerevisiae
T166A kcat/KM for ATP is 37% of wild-type value Saccharomyces cerevisiae
T166S kcat/KM for ATP is 26% of wild-type value Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 ATP pH 7.5, 30°C, wild-type enzyme Saccharomyces cerevisiae
0.51
-
 ATP pH 7.5, 30°C, mutant enzyme T166A Saccharomyces cerevisiae
0.66
-
 ATP pH 7.5, 30°C, mutant enzyme T166S Saccharomyces cerevisiae
0.78
-
 ATP pH 7.5, 30°C, mutant enzyme F162L Saccharomyces cerevisiae
1.1
-
 ATP pH 7.5, 30°C, mutant enzyme Q169E Saccharomyces cerevisiae
3.5
-
 ATP pH 7.5, 30°C, mutant enzyme F162A Saccharomyces cerevisiae
33
-
 ATP pH 7.5, 30°C, mutant enzyme Q169A Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P06634
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O the Q motif regulates ATP binding and hydrolysis, the affinity of the protein for RNA substrates and the helicase activity. At least three different protein conformations that are associated with free, ADP-bound and ATP-bound forms of the protein Saccharomyces cerevisiae ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
DEAD-box RNA helicase
-
 Saccharomyces cerevisiae
DED1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.043
-
 ATP pH 7.5, 30°C, mutant enzyme Q169E Saccharomyces cerevisiae
0.3
-
 ATP pH 7.5, 30°C, mutant enzyme F162A Saccharomyces cerevisiae
1
-
 ATP pH 7.5, 30°C, mutant enzyme Q169A Saccharomyces cerevisiae
1.8
-
 ATP pH 7.5, 30°C, mutant enzyme T166S Saccharomyces cerevisiae
2
-
 ATP pH 7.5, 30°C, mutant enzyme F162L Saccharomyces cerevisiae
2
-
 ATP pH 7.5, 30°C, mutant enzyme T166A Saccharomyces cerevisiae
3.5
-
 ATP pH 7.5, 30°C, wild-type enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.34
-
 ADP pH 7.5, 30°C, mutant enzyme T166S Saccharomyces cerevisiae
0.36
-
 ADP pH 7.5, 30°C, wild-type enzyme Saccharomyces cerevisiae
0.36
-
 ADP pH 7.5, 30°C, mutant enzyme T166A Saccharomyces cerevisiae
3
-
 ADP pH 7.5, 30°C, mutant enzyme F162L Saccharomyces cerevisiae