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Literature summary for 3.6.4.10 extracted from

  • Krajewska, J.; Modrak-Wojcik, A.; Arent, Z.J.; Wieckowski, D.; Zolkiewski, M.; Bzowska, A.; Kedzierska-Mieszkowska, S.
    Characterization of the molecular chaperone ClpB from the pathogenic spirochaete Leptospira interrogans (2017), PLoS ONE, 12, e0181118 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.10

Activating Compound

Activating Compound Comment Organism Structure
kappa-casein the enzyme exhibits a weak ATPase activity that is stimulated about 2fold by kappa-casein Leptospira interrogans
kappa-casein the enzyme exhibits a weak ATPase activity that is stimulated about 4fold by kappa-casein Escherichia coli
additional information aggregated glucose-6-phosphate dehydrogenase does not significantly affect the ATPase activity of the enzyme Escherichia coli
additional information aggregated glucose-6-phosphate dehydrogenase does not significantly affect the ATPase activity of the enzyme Leptospira interrogans
poly-L-lysine the enzyme exhibits a weak ATPase activity that is stimulated about 10fold by poly-L-lysine Escherichia coli
poly-L-lysine the enzyme exhibits a weak ATPase activity that is stimulated about 5fold by poly-L-lysine Leptospira interrogans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells Leptospira interrogans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Escherichia coli
Mg2+ 10 mM used in assay conditions Leptospira interrogans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Escherichia coli
-
 ADP + phosphate
-
 ?
ATP + H2O Leptospira interrogans
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Leptospira interrogans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Co-NTA agarose column chromatography and Superdex S200 gel filtration Escherichia coli
Co-NTA agarose column chromatography and Superdex S200 gel filtration Leptospira interrogans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?
ATP + H2O
-
 Leptospira interrogans ADP + phosphate
-
 ?
ATP + H2O + aggregated fructose-1,6-bisphosphate aldolase
-
 Escherichia coli ADP + phosphate + activated fructose-1,6-bisphosphate aldolase
-
 ?
ATP + H2O + aggregated fructose-1,6-bisphosphate aldolase
-
 Leptospira interrogans ADP + phosphate + activated fructose-1,6-bisphosphate aldolase
-
 ?
ATP + H2O + aggregated glucose-6-phosphate dehydrogenase
-
 Escherichia coli ADP + phosphate + activated glucose-6-phosphate dehydrogenase
-
 ?
ATP + H2O + aggregated glucose-6-phosphate dehydrogenase
-
 Leptospira interrogans ADP + phosphate + activated glucose-6-phosphate dehydrogenase
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Leptospira interrogans

Synonyms

Synonyms Comment Organism
AAA+ chaperone
-
 Escherichia coli
AAA+ chaperone
-
 Leptospira interrogans
ClpB
-
 Escherichia coli
ClpB
-
 Leptospira interrogans
molecular chaperone
-
 Escherichia coli
molecular chaperone
-
 Leptospira interrogans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
67
-
 melting temperature Leptospira interrogans

General Information

General Information Comment Organism
physiological function the enzyme's importance during infection is due to its role as a molecular chaperone involved in reactivation of protein aggregates Escherichia coli
physiological function the enzyme's importance during infection is due to its role as a molecular chaperone involved in reactivation of protein aggregates Leptospira interrogans