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Literature summary for 3.6.4.10 extracted from

  • Dhara, A.; Hussain, M.; Datta, D.; Kumar, M.
    Insights to the assembly of a functionally active leptospiral ClpP1P2 protease complex along with its ATPase chaperone ClpX (2019), ACS Omega, 4, 12880-12895 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leptospira interrogans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.78
-
 ATP at pH 7.8 and 37°C Leptospira interrogans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Leptospira interrogans
-
 ADP + phosphate
-
 ?
ATP + H2O Leptospira interrogans Fiocruz L1-130
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Leptospira interrogans Q72SG5 serovar Copenhageni
-
Leptospira interrogans Fiocruz L1-130 Q72SG5 serovar Copenhageni
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Leptospira interrogans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Leptospira interrogans ADP + phosphate
-
 ?
ATP + H2O
-
 Leptospira interrogans Fiocruz L1-130 ADP + phosphate
-
 ?
ATP + H2O + fluorescein isothiocyanate tagged-casein
-
 Leptospira interrogans ADP + phosphate + ?
-
 ?
ATP + H2O + fluorescein isothiocyanate tagged-casein
-
 Leptospira interrogans Fiocruz L1-130 ADP + phosphate + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Leptospira interrogans

Synonyms

Synonyms Comment Organism
ATPase chaperone
-
 Leptospira interrogans
ClpX Clp ATPase subunit Leptospira interrogans