Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of DnaK /mtHsp70 chimeras by replacing different regions of the DnaK (from Escherichia coli) peptide-binding domain with those of mtHsp70 (from Saccharomyces cerevisiae) results in chimeric proteins that: (a) are not able to support growth of an Escherichia coli DnaK deletion strain at stress temperatures (e.g. 42°C), (b) show increased accessibility and decreased thermal stability of the peptide-binding pocket, and (c) have reduced activation by bacterial, but not mitochondrial cochaperones, as compared with DnaK | Escherichia coli |
additional information | construction of DnaK /mtHsp70 chimeras by replacing different regions of the DnaK (from Escherichia coli) peptide-binding domain with those of mtHsp70 (from Saccharomyces cerevisiae) results in chimeric proteins that: (a) are not able to support growth of an Escherichia coli DnaK deletion strain at stress temperatures (e.g. 42°C), (b) show increased accessibility and decreased thermal stability of the peptide-binding pocket, and (c) have reduced activation by bacterial, but not mitochondrial cochaperones, as compared with DnaK | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DnaK | - |
Escherichia coli |
Hsp70 | - |
Saccharomyces cerevisiae |