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Literature summary for 3.6.4.10 extracted from
- Crystal structure of ClpX molecular chaperone from Helicobacter pylori (2003), J. Biol. Chem., 278, 50664-50670.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of ClpX lacking the N-terminal Cys cluster domain, i.e. residues 71-446 in Escherichia coli | Helicobacter pylori |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| recombinant ClpX lacking the N-terminal Cys cluster domain, i.e. residues 71-446, hanging drop vapor diffusion at 22°C, reservoir solution contains 100 mM imidazole, pH 8.0, 1 M (NH4)2HPO4 and 200 mM NaCl, to obtain crystals of ClpX complexed with nucleotides, 5 mM ADP or ATPgammaS and MgCl2 are added, crystals diffract to 2.6 A | Helicobacter pylori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Helicobacter pylori | - |
ADP + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant ClpX lacking the N-terminal Cys cluster domain, i.e. residues 71-446, Q-Sepharose, Superdex-200 | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Helicobacter pylori | ADP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ClpX | - |
Helicobacter pylori |
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