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Literature summary for 3.6.4.10 extracted from
- Kinetic characterization of the ATPase cycle of the DnaK molecular chaperone (1998), Biochemistry, 37, 596-607.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GrpE | heat shock protein, cochaperonin, increases effectiveness of DnaK 40fold | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| strains carrying plasmid pRLM163 designed for overexpression of a cloned dnaK gene | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000019 | - |
ATP | DnaK | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Bos taurus | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DnaK | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + H2O = ADP + phosphate | highly diverse group of enzymes that perform many functions that are similar to those of chaperonins. They comprise a number of heat-shock-cognate proteins. They are also active in clathrin uncoating and in the oligomerization of actin | Escherichia coli | |
| ATP + H2O = ADP + phosphate | highly diverse group of enzymes that perform many functions that are similar to those of chaperonins. They comprise a number of heat-shock-cognate proteins. They are also active in clathrin uncoating and in the oligomerization of actin | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Bos taurus | ADP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DnaK | - |
Escherichia coli |
| Hsc70 | - |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000333 | - |
ATP | - |
Escherichia coli | |
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