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Literature summary for 3.6.1.B17 extracted from

  • Li, T.F.; Hosmillo, M.; Schwanke, H.; Shu, T.; Wang, Z.; Yin, L.; Curry, S.; Goodfellow, I.G.; Zhou, X.
    Human norovirus NS3 has RNA Helicase and chaperoning activities (2018), J. Virol., 92, e01606-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.B17

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride guanidine hydrochloride inhibits the ATPase activity of the enzyme in a dose-dependent manner, while the addition of guanidine hydrochloride has no obvious effect on enzyme RNA-chaperoning activity Norwalk virus
Mg2+ inhibitory at concentrations higher than 2 mM Norwalk virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the ATPase activity of the enzyme is Mg2+ dependent (optimal at a 2 mM Mg2+) Norwalk virus

Organism

Organism UniProt Comment Textmining
Norwalk virus
-
 genotype GI.1 HuNoV
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Norwalk virus ADP + phosphate
-
 ?
CTP + H2O worst substrate Norwalk virus CDP + phosphate
-
 ?
GTP + H2O best substrate Norwalk virus GDP + phosphate
-
 ?
additional information the enzyme has both ATP-dependent RNA helicase activity that unwinds RNA helices from both 5'->3' and 3'->5' directions and ATP-independent RNA-chaperoning activity that can remodel structured RNAs and facilitate strand annealing. Moreover, the enzyme can facilitate viral RNA synthesis in vitro by norovirus polymerase Norwalk virus ?
-
-
RNA helix + H2O
-
 Norwalk virus ?
-
 ?
UTP + H2O
-
 Norwalk virus UDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
p41
-
 Norwalk virus
protein NS3
-
 Norwalk virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Norwalk virus

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8 the enzyme is active between pH 6.8 and 8.0 Norwalk virus

General Information

General Information Comment Organism
physiological function the enzyme plays an important role in norovirus RNA replication Norwalk virus